ID   REPC_STAAU              Reviewed;         314 AA.
AC   P03064;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Replication initiation protein;
DE            Short=Protein A;
GN   Name=repC;
OS   Staphylococcus aureus.
OG   Plasmid pT181.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6287465; DOI=10.1073/pnas.79.13.4108;
RA   Novick R.P., Adler G.K., Majumder S., Khan S.A., Carleton S.,
RA   Rosenblum W.D., Iordanescu S.;
RT   "Coding sequence for the pT181 repC product: a plasmid-coded protein
RT   uniquely required for replication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:4108-4112(1982).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6657777; DOI=10.1016/0147-619x(83)90039-2;
RA   Khan S.A., Novick R.P.;
RT   "Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid
RT   from Staphylococcus aureus.";
RL   Plasmid 10:251-259(1983).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; J01764; AAA26033.1; -; Genomic_DNA.
DR   PIR; A03600; RQSACT.
DR   RefSeq; NP_040469.1; NC_001393.1.
DR   RefSeq; NP_044359.1; NC_001763.1.
DR   RefSeq; WP_010889937.1; NC_001763.1.
DR   AlphaFoldDB; P03064; -.
DR   SMR; P03064; -.
DR   PRIDE; P03064; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068322"
SQ   SEQUENCE   314 AA;  37657 MW;  F02431AFE570C72F CRC64;
     MYKNNHANHS NHLENHDLDN FSKTGYSNSR LDAHTVCISD PKLSFDAMTI VGNLNRDNAQ
     ALSKFMSVEP QIRLWDILQT KFKAKALQEK VYIEYDKVKA DSWDRRNMRI EFNPNKLTRD
     EMIWLKQNII SYMEDDGFTR LDLAFDFEED LSDYYAMSDK AVKKTIFYGR NGKPETKYFG
     VRDSNRFIRI YNKKQERKDN ADAEVMSEHL WRVEIELKRD MVDYWNDCFS DLHILQPDWK
     TIQRTADRAI VFMLLSDEEE WGKLHRNSRT KYKNLIKEIS PVDLTDLMKS TLKANEKQLQ
     KQIDFWQHEF KFWK