ID   REPD_STAAU              Reviewed;         311 AA.
AC   P03065;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Replication initiation protein;
GN   Name=repD;
OS   Staphylococcus aureus.
OG   Plasmid pC221.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3860383; DOI=10.1002/j.1460-2075.1985.tb03665.x;
RA   Brenner D.G., Shaw W.V.;
RT   "The use of synthetic oligonucleotides with universal templates for rapid
RT   DNA sequencing: results with staphylococcal replicon pC221.";
RL   EMBO J. 4:561-568(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2993795; DOI=10.1007/bf00330758;
RA   Projan S.J., Kornblum J., Moghazeh S.L., Edelman I., Gennaro M.L.,
RA   Novick R.P.;
RT   "Comparative sequence and functional analysis of pT181 and pC221, cognate
RT   plasmid replicons from Staphylococcus aureus.";
RL   Mol. Gen. Genet. 199:452-464(1985).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
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DR   EMBL; X02166; CAA26104.1; -; Genomic_DNA.
DR   EMBL; X02529; CAA26366.1; -; Genomic_DNA.
DR   PIR; A03601; RQSAD2.
DR   RefSeq; NP_052693.1; NC_002129.1.
DR   RefSeq; WP_002489526.1; NZ_NADF01000039.1.
DR   RefSeq; YP_001595585.1; NC_010111.1.
DR   RefSeq; YP_232726.1; NC_006977.1.
DR   PDB; 4CWC; X-ray; 2.90 A; A/C=32-226.
DR   PDB; 4CWE; X-ray; 3.00 A; A/C=32-226.
DR   PDBsum; 4CWC; -.
DR   PDBsum; 4CWE; -.
DR   AlphaFoldDB; P03065; -.
DR   SMR; P03065; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA replication; Plasmid.
FT   CHAIN           1..311
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068323"
FT   STRAND          39..50
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           53..65
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          69..73
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          76..83
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   TURN            84..87
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          88..93
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           94..100
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          105..109
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           111..113
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           116..125
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           127..129
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          131..146
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          151..157
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          161..165
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          169..177
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          179..189
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           190..196
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          203..205
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          208..215
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           216..224
FT                   /evidence="ECO:0007829|PDB:4CWC"
SQ   SEQUENCE   311 AA;  37479 MW;  BE050FBD26387348 CRC64;
     MSTENHSNYL QNKDLDNFSK TGYSNSRLSG NFFTTPQPEL SFDAMTIVGN LNKTNAKKLS
     DFMSTEPQIR LWDILQTKFK AKALQEKVYI EYDKVKADSW DRRNMRVEFN PNKLTHEEML
     WLKQNIIDYM EDDGFTRLDL AFDFEDDLSD YYAMTDKAVK KTIFYGRNGK PETKYFGVRD
     SDRFIRIYNK KQERKDNADV EVMSEHLWRV EIELKRDMVD YWNDCFDDLH ILKPDWTTPE
     KVKEQAMVYL LLNEEGTWGK LERHAKYKYK QLIKEISPID LTELMKSTLK ENEKQLQKQI
     DFWQREFRFW K