REPE1_ECOLI
ID REPE1_ECOLI Reviewed; 251 AA.
AC P03856; P08969;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Replication initiation protein;
DE Short=Protein E;
DE Short=Protein rep;
DE AltName: Full=Protein F4;
GN Name=repE; Synonyms=E, rep; OrderedLocusNames=ECOK12F045;
OS Escherichia coli (strain K12).
OG Plasmid F.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7028572; DOI=10.1016/0378-1119(81)90135-9;
RA Murotsu T., Matsubara K., Sugisaki H., Takanami M.;
RT "Nine unique repeating sequences in a region essential for replication and
RT incompatibility of the mini-F plasmid.";
RL Gene 15:257-271(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Eichenlaub R.;
RT "F plasmid DNA complete mini-F region (F coordinates 40.301F to 49.869F).";
RL Submitted (AUG-1986) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / CR63;
RA Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT "Complete nucleotide sequence of the F plasmid: its implications for
RT organization and diversification of plasmid genomes.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-251.
RX PubMed=7018695; DOI=10.1016/0092-8674(81)90095-7;
RA Tolun A., Helinski D.R.;
RT "Direct repeats of the F plasmid incC region express F incompatibility.";
RL Cell 24:687-694(1981).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RX PubMed=3007930; DOI=10.1007/bf00330529;
RA Disque-Kochem C., Seidel U., Helsberg M., Eichenlaub R.;
RT "The repeated sequences (incB) preceding the protein E gene of plasmid
RT mini-F are essential for replication.";
RL Mol. Gen. Genet. 202:132-135(1986).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 209-251.
RC STRAIN=K12;
RX PubMed=3029390; DOI=10.1016/0022-2836(86)90459-6;
RA Mori H., Kondo A., Ohshima A., Ogura T., Hiraga S.;
RT "Structure and function of the F plasmid genes essential for
RT partitioning.";
RL J. Mol. Biol. 192:1-15(1986).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH DNA.
RX PubMed=10469640; DOI=10.1093/emboj/18.17.4597;
RA Komori H., Matsunaga F., Higuchi Y., Ishiai M., Wada C., Miki K.;
RT "Crystal structure of a prokaryotic replication initiator protein bound to
RT DNA at 2.6 A resolution.";
RL EMBO J. 18:4597-4607(1999).
CC -!- FUNCTION: Replication initiator in the monomeric form, and autogenous
CC repressor in the dimeric form.
CC -!- SUBUNIT: Monomer and homodimer. {ECO:0000269|PubMed:10469640}.
CC -!- SIMILARITY: Belongs to the initiator RepB protein family.
CC {ECO:0000305}.
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DR EMBL; J01724; AAA91062.1; -; Genomic_DNA.
DR EMBL; M12987; AAA24901.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97915.1; -; Genomic_DNA.
DR EMBL; X03410; CAA27146.1; -; Genomic_DNA.
DR EMBL; X04619; CAA28294.1; -; Genomic_DNA.
DR PIR; A91487; QQECF.
DR RefSeq; NP_061424.1; NC_002483.1.
DR RefSeq; WP_000852146.1; NZ_STEB01000056.1.
DR PDB; 1REP; X-ray; 2.60 A; C=1-251.
DR PDB; 2Z9O; X-ray; 3.14 A; A/B=2-251.
DR PDB; 7RVA; X-ray; 1.89 A; C=1-251.
DR PDBsum; 1REP; -.
DR PDBsum; 2Z9O; -.
DR PDBsum; 7RVA; -.
DR AlphaFoldDB; P03856; -.
DR SMR; P03856; -.
DR DIP; DIP-27645N; -.
DR PRIDE; P03856; -.
DR PhylomeDB; P03856; -.
DR EvolutionaryTrace; P03856; -.
DR PRO; PR:P03856; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR000525; Initiator_Rep_prot.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF01051; Rep_3; 1.
DR SUPFAM; SSF46785; SSF46785; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; DNA-binding; Plasmid; Plasmid copy control.
FT CHAIN 1..251
FT /note="Replication initiation protein"
FT /id="PRO_0000068297"
FT DNA_BIND 75..83
FT /note="H-T-H motif"
FT DNA_BIND 200..207
FT /note="H-T-H motif"
FT REGION 1..144
FT /note="Dimerization"
FT STRAND 17..21
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 22..25
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 33..47
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 58..63
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 64..71
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 75..88
FT /evidence="ECO:0007829|PDB:7RVA"
FT TURN 89..91
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 110..123
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 126..131
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 136..140
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 160..171
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 185..192
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 196..199
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 201..207
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 209..219
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 220..231
FT /evidence="ECO:0007829|PDB:7RVA"
FT STRAND 234..244
FT /evidence="ECO:0007829|PDB:7RVA"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:2Z9O"
SQ SEQUENCE 251 AA; 29359 MW; EA2BC265D80158B2 CRC64;
MAETAVINHK KRKNSPRIVQ SNDLTEAAYS LSRDQKRMLY LFVDQIRKSD GTLQEHDGIC
EIHVAKYAEI FGLTSAEASK DIRQALKSFA GKEVVFYRPE EDAGDEKGYE SFPWFIKRAH
SPSRGLYSVH INPYLIPFFI GLQNRFTQFR LSETKEITNP YAMRLYESLC QYRKPDGSGI
VSLKIDWIIE RYQLPQSYQR MPDFRRRFLQ VCVNEINSRT PMRLSYIEKK KGRQTTHIVF
SFRDITSMTT G
