ID   REPE_STAAU              Reviewed;         314 AA.
AC   P12053;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Replication initiation protein;
GN   Name=repE;
OS   Staphylococcus aureus.
OG   Plasmid pS194.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3357770; DOI=10.1093/nar/16.5.2179;
RA   Projan S.J., Moghazeh S., Novick R.P.;
RT   "Nucleotide sequence of pS194, a streptomycin-resistance plasmid from
RT   Staphylococcus aureus.";
RL   Nucleic Acids Res. 16:2179-2187(1988).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- MISCELLANEOUS: PS194 is a streptomycin-resistance plasmid.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
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DR   EMBL; X06627; CAA29842.1; -; Genomic_DNA.
DR   PIR; S00937; S00937.
DR   RefSeq; NP_976274.1; NC_005564.1.
DR   RefSeq; WP_011167206.1; NZ_WNWJ01000020.1.
DR   PDB; 4CWC; X-ray; 2.90 A; A/C=220-314.
DR   PDBsum; 4CWC; -.
DR   AlphaFoldDB; P12053; -.
DR   SMR; P12053; -.
DR   PRIDE; P12053; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068324"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          230..237
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           239..241
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           245..256
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           258..261
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           266..279
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   STRAND          280..283
FT                   /evidence="ECO:0007829|PDB:4CWC"
FT   HELIX           284..307
FT                   /evidence="ECO:0007829|PDB:4CWC"
SQ   SEQUENCE   314 AA;  38086 MW;  E8ECE1DE9EF2BFB8 CRC64;
     MSKKAEEIQA KQSLEKENSN FSKTGYSNSR LNRHIMYTPE PKLHFDAMTI VGNLNKNNAH
     KLSEFMSIAP QIRLWDILQT KFKAKALQEK VYIEYDKVKA DAWDRRNMRV EFNPNKLTHE
     EMLWLKQNII DYMEDDGFTR LDLAFDFEDD LSDYYAMTDK SVKKTIFYGR NGKPETKYFG
     VRDSDRFIRI YNKKQERKDN ADIEVMSEHL WRVEIELKRD MVDYWNDCFN DLHILKPDWS
     SLEKVKDQAM IYMLIHEEST WGKLERRTKN KYREMLKSIS EIDLTDLMKL TLKENEKQLQ
     KQIEFWQREF RFWE