ID   REPJ_ECOLX              Reviewed;         279 AA.
AC   P20356;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Regulatory protein RepA;
GN   Name=repA;
OS   Escherichia coli.
OG   Plasmid IncQ RSF1010.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2653965; DOI=10.1016/0378-1119(89)90273-4;
RA   Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M.,
RA   Scherzinger E.;
RT   "Complete nucleotide sequence and gene organization of the broad-host-range
RT   plasmid RSF1010.";
RL   Gene 75:271-288(1989).
CC   -!- FUNCTION: This protein is involved in regulating the plasmid copy-
CC       number. Increasing the level of this protein results in a higher
CC       plasmid copy-number.
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DR   EMBL; M28829; AAA26450.1; -; Genomic_DNA.
DR   PIR; JH0130; RGECRI.
DR   RefSeq; NP_044309.1; NC_001740.1.
DR   RefSeq; WP_000190708.1; NZ_VTMX01000009.1.
DR   PDB; 1G8Y; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J/K/L=1-279.
DR   PDB; 1NLF; X-ray; 1.95 A; A/B/C=1-279.
DR   PDB; 1OLO; X-ray; 2.55 A; A/B=1-279.
DR   PDBsum; 1G8Y; -.
DR   PDBsum; 1NLF; -.
DR   PDBsum; 1OLO; -.
DR   AlphaFoldDB; P20356; -.
DR   SMR; P20356; -.
DR   DIP; DIP-59501N; -.
DR   IntAct; P20356; 1.
DR   BRENDA; 3.6.4.12; 2026.
DR   EvolutionaryTrace; P20356; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   CDD; cd01125; repA; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038724; RepA.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Nucleotide-binding; Plasmid;
KW   Plasmid copy control.
FT   CHAIN           1..279
FT                   /note="Regulatory protein RepA"
FT                   /id="PRO_0000068333"
FT   HELIX           9..14
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          22..24
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          31..38
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           43..55
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          71..78
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           80..91
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           96..105
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          106..108
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   TURN            112..114
FT                   /evidence="ECO:0007829|PDB:1OLO"
FT   HELIX           121..131
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          135..140
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           142..145
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           153..170
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          173..179
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           203..205
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          209..214
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           217..222
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          227..229
FT                   /evidence="ECO:0007829|PDB:1G8Y"
FT   HELIX           230..232
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          233..240
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          242..245
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          250..254
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   HELIX           256..258
FT                   /evidence="ECO:0007829|PDB:1NLF"
FT   STRAND          260..262
FT                   /evidence="ECO:0007829|PDB:1NLF"
SQ   SEQUENCE   279 AA;  29909 MW;  69CD06FF8D3FFBB2 CRC64;
     MATHKPINIL EAFAAAPPPL DYVLPNMVAG TVGALVSPGG AGKSMLALQL AAQIAGGPDL
     LEVGELPTGP VIYLPAEDPP TAIHHRLHAL GAHLSAEERQ AVADGLLIQP LIGSLPNIMA
     PEWFDGLKRA AEGRRLMVLD TLRRFHIEEE NASGPMAQVI GRMEAIAADT GCSIVFLHHA
     SKGAAMMGAG DQQQASRGSS VLVDNIRWQS YLSSMTSAEA EEWGVDDDQR RFFVRFGVSK
     ANYGAPFADR WFRRHDGGVL KPAVLERQRK SKGVPRGEA