ID   REPM_STAAU              Reviewed;         314 AA.
AC   P14490;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Replication initiation protein;
GN   Name=repM;
OS   Staphylococcus aureus.
OG   Plasmid pC223.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RN154;
RX   PubMed=3128771; DOI=10.1093/nar/16.5.2045;
RA   Ehret M., Matzura H.;
RT   "Replication control of the Staphylococcus aureus chloramphenicol
RT   resistance plasmids pC223 and pUB112 in Bacillus subtilis.";
RL   Nucleic Acids Res. 16:2045-2062(1988).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
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DR   EMBL; X07371; CAA30291.1; -; Genomic_DNA.
DR   PIR; S00909; S00909.
DR   AlphaFoldDB; P14490; -.
DR   SMR; P14490; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068325"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   314 AA;  37550 MW;  3590136CF17C361E CRC64;
     MSKNNYTNHS NHLENHDSDN FSKTGYSNSR LDAHFVCTSN PKLSFDAMTI VGNLNKNSAK
     KLSDFMSLDP QIRLWDILQT KFKAKALQEK VYIEYDKVKA DTWDRRNMRV EFNPNKLTHD
     EMLWLKHNII DYMEDDGFTR LDLAFDFEDD LSDYYALSEK ALKRTVFFGT TGKAETKYFG
     SRDSNRFIRI YNKKKERKEN ADVDVSAEHL WRVEIELKRD MVDYWNNCFN DLHILKPAWA
     TLESLKEQAM VYLLLHEESK WGELHRNSRR KYKQIIQEIS SIDLTDLMKS TLTDNEENLQ
     KQINFWQRKF EFWK