ID   REPN_STAAU              Reviewed;         314 AA.
AC   P19529;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Replication initiation protein;
GN   Name=repN;
OS   Staphylococcus aureus.
OG   Plasmid pCW7.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2270231; DOI=10.1016/0147-619x(90)90027-a;
RA   Balson D.F., Shaw W.V.;
RT   "Nucleotide sequence of the rep gene of staphylococcal plasmid pCW7.";
RL   Plasmid 24:74-80(1990).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
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DR   EMBL; J03323; AAA26669.1; -; Genomic_DNA.
DR   PIR; A37389; A37389.
DR   RefSeq; WP_017431565.1; NZ_SDOB01000031.1.
DR   PDB; 4CWE; X-ray; 3.00 A; A/C=220-314.
DR   PDBsum; 4CWE; -.
DR   AlphaFoldDB; P19529; -.
DR   SMR; P19529; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068326"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          230..235
FT                   /evidence="ECO:0007829|PDB:4CWE"
FT   HELIX           239..241
FT                   /evidence="ECO:0007829|PDB:4CWE"
FT   HELIX           245..256
FT                   /evidence="ECO:0007829|PDB:4CWE"
FT   HELIX           258..263
FT                   /evidence="ECO:0007829|PDB:4CWE"
FT   HELIX           266..279
FT                   /evidence="ECO:0007829|PDB:4CWE"
FT   HELIX           285..307
FT                   /evidence="ECO:0007829|PDB:4CWE"
SQ   SEQUENCE   314 AA;  38163 MW;  F42A3AAFC37BFA4C CRC64;
     MSKNNHANHS NHLENHDLDN FSKTGYSNSR LNRHTMYTPE PKLSFDAMTI VGNLNKNNAH
     KLSEFMSVEP QIRLWDILQT KFKAKALQEK VYIEYDKVKA DTWDRRNMRV EFNPNKLTHE
     EMLWLKQNII DYMEDDGFTR LDLAFDFEYD LSDYYAMTDK SVKKTIFYGR NGKPETKYFG
     VRDSDRFIRI YNKKQERKDN ADIKIMSEHL WRVEIELKRD MVDYWNDCFN DLHILQPDWK
     TIERTSDRAM VFMLLNDEEE WGKLERRTKN KYKKLIKEIS LIDLTDLMKS TLKANEKQLQ
     KQIDFWQREF RFWK