ID   REPS_BACAN              Reviewed;         512 AA.
AC   Q9RMZ4; Q7CMA0;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Replication initiation protein;
GN   Name=repS; Synonyms=repA;
GN   OrderedLocusNames=pXO2-38, BXB0039, GBAA_pXO2_0039;
OS   Bacillus anthracis.
OG   Plasmid pXO2.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sm;
RA   Uchida I., Makino S., Nichimori K.;
RT   "Cloning and characterization of replication origin of capsule plasmid
RT   pXO2.";
RL   Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Pasteur;
RX   PubMed=10475962; DOI=10.1046/j.1365-2672.1999.00883.x;
RA   Okinaka R.T., Cloud K., Hampton O., Hoffmaster A., Hill K.K., Keim P.,
RA   Koehler T., Lamke G., Kumano S., Manter D., Martinez Y., Ricke D.,
RA   Svensson R., Jackson P.J.;
RT   "Sequence, assembly and analysis of pXO1 and pXO2.";
RL   J. Appl. Microbiol. 87:261-262(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ames / isolate Florida / A2012;
RX   PubMed=12004073; DOI=10.1126/science.1071837;
RA   Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA   Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA   Fraser C.M.;
RT   "Comparative genome sequencing for discovery of novel polymorphisms in
RT   Bacillus anthracis.";
RL   Science 296:2028-2033(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [5]
RP   FUNCTION.
RC   STRAIN=9131;
RX   PubMed=15090513; DOI=10.1128/jb.186.9.2717-2723.2004;
RA   Tinsley E., Naqvi A., Bourgogne A., Koehler T.M., Khan S.A.;
RT   "Isolation of a minireplicon of the virulence plasmid pXO2 of Bacillus
RT   anthracis and characterization of the plasmid-encoded RepS replication
RT   protein.";
RL   J. Bacteriol. 186:2717-2723(2004).
CC   -!- FUNCTION: Essential for replication. Binds specifically to a 60-bp
CC       region corresponding to the putative origin of replication of pXO2.
CC       Binds also nonspecifically to single-stranded DNA with lower affinity.
CC       {ECO:0000269|PubMed:15090513}.
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DR   EMBL; AB017611; BAB82449.1; -; Genomic_DNA.
DR   EMBL; AF188935; AAF13643.1; -; Genomic_DNA.
DR   EMBL; AE011191; AAM26199.1; -; Genomic_DNA.
DR   EMBL; AE017335; AAT28969.2; -; Genomic_DNA.
DR   RefSeq; NP_053193.1; NC_002146.1.
DR   RefSeq; WP_001099042.1; NZ_VTZL01000009.1.
DR   AlphaFoldDB; Q9RMZ4; -.
DR   IntAct; Q9RMZ4; 1.
DR   EnsemblBacteria; AAT28969; AAT28969; GBAA_pXO2_0039.
DR   GeneID; 45025349; -.
DR   KEGG; bar:GBAA_pXO2_0039; -.
DR   HOGENOM; CLU_042279_0_0_9; -.
DR   OMA; TCNHFGI; -.
DR   Proteomes; UP000000594; Plasmid pXO2.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR014820; PriCT_1.
DR   Pfam; PF08708; PriCT_1; 1.
PE   4: Predicted;
KW   DNA replication; DNA-binding; Plasmid; Reference proteome.
FT   CHAIN           1..512
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068334"
SQ   SEQUENCE   512 AA;  58152 MW;  108A932FC4FCA93A CRC64;
     MNTVQKAIEL ILHKGLRKYK SKNSKAGLVS IANQEKFESK VLNGKKNKKG SIFITRKKED
     LRAKFGTRGV VLSSEEAVLD HVGQASHWTP NVYNFGTYGQ NGLRTIVGHT EKNLQQINCF
     VIDIDSKSFP MTAINDVALN AGFGVPTMIL ETTKGYQVYY VLDKAVYVSN NKNFIAIKSA
     KRISQNLREM FADSLPNVDL TCNHFGFFRM PSVQNIVMFF EENVYTFKEL QEWSKRQDDN
     KGNEQFHNVI ESPFAKNAPV EQPKQMDELW FKQVISCTNV SPKQTKAGRN NAIFTLSLAC
     FQSQYAIKDT MDLMDQFNSN LEQPLEHTEV RGIVMSAYSG KYQAAHKDYI ERLLQTYGMG
     QASAFRAPSV LWKKHKKQRK DRVRSHWHEW EADIITFLSM NSNNKPVLYF TQSELCEALN
     VPRSTLNTVL KKSTKIYKTV EGKGKTAKTG LSTLGMLIAF ALKENGKRRE SYLNYLQGLF
     PKTGNILEQA KTSNVMEEQE TLYGILEGLP AG