ID   REPX_STAAU              Reviewed;         229 AA.
AC   P03862;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Protein rep;
DE   AltName: Full=Reading frame A;
DE   AltName: Full=Replication protein;
OS   Staphylococcus aureus.
OG   Plasmid pC194.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6950931; DOI=10.1128/jb.150.2.815-825.1982;
RA   Horinouchi S., Weisblum B.;
RT   "Nucleotide sequence and functional map of pC194, a plasmid that specifies
RT   inducible chloramphenicol resistance.";
RL   J. Bacteriol. 150:815-825(1982).
CC   -!- FUNCTION: Produces a single-strand nick in a specific site of the
CC       plasmid, and this nick results in single-strand replication by rolling
CC       circle mechanism.
CC   -!- MISCELLANEOUS: PC194 is a plasmid that specifies inducible
CC       chloramphenicol resistance.
CC   -!- SIMILARITY: Belongs to the Gram-positive plasmids replication protein
CC       type 1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA24585.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; V01277; CAA24585.1; ALT_INIT; Genomic_DNA.
DR   PIR; A04491; QQSAEC.
DR   RefSeq; NP_040435.3; NC_002013.1.
DR   RefSeq; WP_010890867.1; NC_002013.1.
DR   AlphaFoldDB; P03862; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR000989; Rep.
DR   Pfam; PF01446; Rep_1; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..229
FT                   /note="Protein rep"
FT                   /id="PRO_0000068319"
FT   BINDING         214
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   229 AA;  27546 MW;  D0FF587D2BEA18C4 CRC64;
     MCYNMEKYTE KKQRNQVFQK FIKRHIGENQ MDLVEDCNTF LSFVADKTLE KQKLYKANSC
     KNRFCPVCAW RKARKDALGL SLMMQYIKQQ EKKEFIFLTL TTPNVMSDEL ENEIKRYNNS
     FRKLIKRKKV GSVIKGYVRK LEITYNKKRD DYNPHFHVLI AVNKSYFTDK RYYISQQEWL
     DLWRDVTGIS EITQVQVQKI RQNNNKELYE MAKYSGKDSD YLINKSKSL