ID   REPY_STAAU              Reviewed;         199 AA.
AC   P03858;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Replication protein;
GN   Name=repF; Synonyms=repC;
OS   Staphylococcus aureus.
OG   Plasmid pE194.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2443486; DOI=10.1128/jb.169.10.4822-4829.1987;
RA   Villafane R., Bechhofer D.H., Narayanan C.S., Dubnau D.;
RT   "Replication control genes of plasmid pE194.";
RL   J. Bacteriol. 169:4822-4829(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6279574; DOI=10.1128/jb.150.2.804-814.1982;
RA   Horinouchi S., Weisblum B.;
RT   "Nucleotide sequence and functional map of pE194, a plasmid that specifies
RT   inducible resistance to macrolide, lincosamide, and streptogramin type B
RT   antibodies.";
RL   J. Bacteriol. 150:804-814(1982).
CC   -!- FUNCTION: Is essential for plasmid replication. Nicks the positive
CC       strand at the plus origin of replication.
CC   -!- SIMILARITY: Belongs to the Gram-positive plasmids replication protein
CC       type 2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA24589.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M17811; AAA25603.1; -; Genomic_DNA.
DR   EMBL; V01278; CAA24589.1; ALT_FRAME; Genomic_DNA.
DR   PIR; A04487; QQSABE.
DR   RefSeq; WP_011178346.1; NC_005908.1.
DR   RefSeq; WP_012816703.1; NZ_VRQI01000067.1.
DR   RefSeq; YP_006938614.1; NC_013345.1.
DR   RefSeq; YP_009060504.1; NC_024964.1.
DR   RefSeq; YP_025319.1; NC_005908.1.
DR   AlphaFoldDB; P03858; -.
DR   SMR; P03858; -.
DR   GO; GO:0005727; C:extrachromosomal circular DNA; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR002631; Plasmid_rep.
DR   Pfam; PF01719; Rep_2; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..199
FT                   /note="Replication protein"
FT                   /id="PRO_0000068330"
SQ   SEQUENCE   199 AA;  23752 MW;  56B08269324807A3 CRC64;
     MSENVIKETE NKKNSRGRNW TFVLYPESAK AEWLEYLKEL HIQFVVSPLH DRDTDTEGRM
     KKEHYHILVM YEGNKSYEQI KIITEELNAT IPQIAGSVKG LVRYMLHMDD PNKFKYQKED
     MIVYGGVDVD ELLKKTTTDR YKLIKEMIEF IDEQGIVEFK SLMDYAMKFK FDDWFPLLCD
     NSAYVIQEYI KSNRYKSDR