ID   REP_BPPF3               Reviewed;         278 AA.
AC   P03627;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Replication-associated protein G2P;
DE            Short=Rep;
DE            EC=3.1.21.-;
DE            EC=6.5.1.1;
DE   AltName: Full=G2P;
DE   AltName: Full=Gene 2 protein;
GN   Name=II;
OS   Pseudomonas phage Pf3 (Bacteriophage Pf3).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; Tertilicivirus.
OX   NCBI_TaxID=10872;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=New York, and Nijmegen;
RX   PubMed=3928901; DOI=10.1128/jvi.56.1.268-276.1985;
RA   Luiten R.G.M., Putterman D.G., Schoenmakers J.G.G., Konings R.N.H.,
RA   Day L.A.;
RT   "Nucleotide sequence of the genome of Pf3, an IncP-1 plasmid-specific
RT   filamentous bacteriophage of Pseudomonas aeruginosa.";
RL   J. Virol. 56:268-276(1985).
CC   -!- FUNCTION: Isoform G2P plays an essential role in viral DNA replication.
CC       Binds the origin of replication and cleaves the dsDNA replicative form
CC       I (RFI) and becomes covalently bound to it via phosphotyrosine bond,
CC       generating the dsDNA replicative form II (RFII). In turn, viral DNA
CC       replication initiates at the 3'-OH of the cleavage site. After one
CC       round of rolling circle synthesis, protein G2P is linked to the newly
CC       synthesized ssDNA and joins the ends of the displaced strand to
CC       generate a circular single-stranded molecule ready to be packed into a
CC       virion. {ECO:0000250}.
CC   -!- FUNCTION: Isoform G10P protein binds to double-stranded DNA and
CC       prevents hydrolysis by nucleases. Additionally, G10P is an inhibitor of
CC       DNA replication and may have a role in the transition from
CC       semiconservative replicative form DNA replication to single-stranded
CC       DNA synthesis in the life cycle. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC         diphosphate.; EC=6.5.1.1;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=G2P; Synonyms=Gene 2 protein;
CC         IsoId=P03627-1; Sequence=Displayed;
CC       Name=G10P; Synonyms=Gene 10 protein;
CC         IsoId=P03627-2; Sequence=VSP_037574;
CC   -!- SIMILARITY: Belongs to the inovirus G2P protein family. {ECO:0000305}.
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DR   EMBL; M11912; AAA88383.1; -; Genomic_DNA.
DR   EMBL; M19377; AAA88392.1; -; Genomic_DNA.
DR   PIR; A04235; Z2BP73.
DR   RefSeq; NP_040657.1; NC_001418.1. [P03627-1]
DR   GeneID; 1260909; -.
DR   KEGG; vg:1260909; -.
DR   Proteomes; UP000001719; Genome.
DR   Proteomes; UP000009090; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Alternative initiation; DNA replication; DNA-binding; Endonuclease;
KW   Hydrolase; Ligase; Nuclease; Reference proteome.
FT   CHAIN           1..278
FT                   /note="Replication-associated protein G2P"
FT                   /id="PRO_0000098210"
FT   VAR_SEQ         1..205
FT                   /note="Missing (in isoform G10P)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_037574"
FT   VARIANT         77
FT                   /note="Y -> H (in strain: New York)"
SQ   SEQUENCE   278 AA;  32134 MW;  40D6167E997ABB71 CRC64;
     MRCLKSFRHG FTLGTNCYEP GAKRPRGDRS TVGGWSHGST SRNIAFLRSI DEAKLTGHAL
     ALTLTVRDCP DTSDDWYKVR RAFEMRLRRL GLIRMHWVIE WQRRGVPHLH CAAFFDDTAP
     ALLPAVIMRS WCDLVSDYNA SPLSQYVLPI TDAIGWFQYV SKHAARGVNH YQRSPENIPP
     QWQKKTGRMW GKVGEWPVVE KAEIFMGDAT FYQLRRIVKR WRFADARASG SIHRIRSAKK
     YLQVPETLSR VLGASEWMPE NELLSILYFL KSQGYEFL