ID   REP_BPPHL               Reviewed;         346 AA.
AC   Q38617; Q76TM0;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Replication-associated protein G2P;
DE            Short=Rep;
DE            EC=3.1.21.-;
DE            EC=6.5.1.1;
DE   AltName: Full=G2P;
DE   AltName: Full=Gene 2 protein;
GN   Name=II;
OS   Xanthomonas phage phiLf (Bacteriophage phi-Lf).
OC   Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes;
OC   Tubulavirales; Inoviridae; unclassified Inoviridae.
OX   NCBI_TaxID=28365;
OH   NCBI_TaxID=340; Xanthomonas campestris pv. campestris.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8573116; DOI=10.1006/bbrc.1996.0003;
RA   Lin N.T., Wen F.S., Tseng Y.H.;
RT   "A region of the filamentous phage phi Lf genome that can support
RT   autonomous replication and miniphage production.";
RL   Biochem. Biophys. Res. Commun. 218:12-16(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8113723; DOI=10.1099/0022-1317-75-1-15;
RA   Wen F.-S., Tseng Y.-H.;
RT   "Nucleotide sequence determination, characterization and purification of
RT   the single-stranded DNA-binding protein and major coat protein of
RT   filamentous phage phi-Lf of Xanthomonas campestris pv. campestris.";
RL   J. Gen. Virol. 75:15-22(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7789813; DOI=10.1016/0378-1119(95)00170-b;
RA   Shieh G.J., Lin C.H., Kuo J.L., Kuo T.T.;
RT   "Characterization of an open reading frame involved in site-specific
RT   integration of filamentous phage Cf1t from Xanthomonas campestris pv.
RT   citri.";
RL   Gene 158:73-76(1995).
CC   -!- FUNCTION: Isoform G2P plays an essential role in viral DNA replication.
CC       Binds the origin of replication and cleaves the dsDNA replicative form
CC       I (RFI) and becomes covalently bound to it via phosphotyrosine bond,
CC       generating the dsDNA replicative form II (RFII). In turn, viral DNA
CC       replication initiates at the 3'-OH of the cleavage site. After one
CC       round of rolling circle synthesis, protein G2P is linked to the newly
CC       synthesized ssDNA and joins the ends of the displaced strand to
CC       generate a circular single-stranded molecule ready to be packed into a
CC       virion. {ECO:0000250}.
CC   -!- FUNCTION: Isoform G10P protein binds to double-stranded DNA and
CC       prevents hydrolysis by nucleases. Additionally, G10P is an inhibitor of
CC       DNA replication and may have a role in the transition from
CC       semiconservative replicative form DNA replication to single-stranded
CC       DNA synthesis in the life cycle. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC         diphosphate.; EC=6.5.1.1;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=G2P; Synonyms=Gene 2 protein;
CC         IsoId=Q38617-1; Sequence=Displayed;
CC       Name=G10P; Synonyms=Gene 10 protein;
CC         IsoId=Q38617-2; Sequence=VSP_037577;
CC   -!- SIMILARITY: Belongs to the inovirus G2P protein family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC54631.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U38235; AAC54630.1; -; Genomic_DNA.
DR   EMBL; U38235; AAC54631.1; ALT_INIT; Genomic_DNA.
DR   PRIDE; Q38617; -.
DR   Proteomes; UP000007611; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Alternative initiation; DNA replication; DNA-binding; Endonuclease;
KW   Hydrolase; Ligase; Nuclease; Reference proteome.
FT   CHAIN           1..346
FT                   /note="Replication-associated protein G2P"
FT                   /id="PRO_0000378354"
FT   VAR_SEQ         1..181
FT                   /note="Missing (in isoform G10P)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_037577"
SQ   SEQUENCE   346 AA;  39173 MW;  B9A50F9BD511C193 CRC64;
     MADGTCSFCG EPTVYFFPGG LCVSCTSKNA RIRMQEQPTQ SRELSAFDAS VGVMQAATRR
     TEIAAEKIQK NKRVVGTSVR EFDAAHPIAL TPEGQRAALA LGLVHYKTSD TRASTTGTVT
     IEIDPLQARA QRLRKSVITG ARLHDQEAKK GSFRGAWYFL TLTYRDGSDS SPRDVSELFK
     RMRGHFNRLK SGRARWNRES FRYVWVGELT QRFRPHYHVM LWVPQGMFFG KVDQRGWWPH
     GSSQIEKARN CVGYLAKYAS KFTAITAAAF PKGFRTHGCG GLNTESKREL RWWKAPKDAR
     EALGGEADIR KAKGGWFDRL TGEFWPSPWK VTFIFGRTFA WKVVQL