ID   REP_BUCAI               Reviewed;         645 AA.
AC   P57654;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=ATP-dependent DNA helicase Rep {ECO:0000255|HAMAP-Rule:MF_01920};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01920};
GN   Name=rep {ECO:0000255|HAMAP-Rule:MF_01920}; OrderedLocusNames=BU598;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Rep helicase is a single-stranded DNA-dependent ATPase
CC       involved in DNA replication; it can initiate unwinding at a nick in the
CC       DNA. It binds to the single-stranded DNA and acts in a progressive
CC       fashion along the DNA in the 3' to 5' direction. {ECO:0000255|HAMAP-
CC       Rule:MF_01920}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01920};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01920}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01920}.
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DR   EMBL; BA000003; BAB13283.1; -; Genomic_DNA.
DR   RefSeq; NP_240397.1; NC_002528.1.
DR   RefSeq; WP_009874546.1; NC_002528.1.
DR   AlphaFoldDB; P57654; -.
DR   SMR; P57654; -.
DR   STRING; 107806.10039249; -.
DR   PRIDE; P57654; -.
DR   EnsemblBacteria; BAB13283; BAB13283; BAB13283.
DR   KEGG; buc:BU598; -.
DR   PATRIC; fig|107806.10.peg.601; -.
DR   eggNOG; COG0210; Bacteria.
DR   HOGENOM; CLU_004585_5_2_6; -.
DR   OMA; HCANILI; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_01920; Helicase_Rep; 1.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR005752; Helicase_Rep.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01074; rep; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..645
FT                   /note="ATP-dependent DNA helicase Rep"
FT                   /id="PRO_0000102065"
FT   DOMAIN          1..280
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   DOMAIN          281..562
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   BINDING         22..29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   BINDING         278
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
SQ   SEQUENCE   645 AA;  75814 MW;  8C1B425B122D3224 CRC64;
     MSLNFSQKNA VELTNGPCLI LAGAGSGKTK VIINKIIYLI NHCQYEPDNI AAVTFTNKAA
     YEMRIRLSKY LNIPEIKKII ISTFHSLGLE IIKKEIDALE LNNNFTLLDE KDQILLLKKI
     CKKEIKNNIQ LLKKLNFMIS YWKNKFLTPL QVQLLAKSSQ EKDFAYVYEQ YTNYLYKANI
     LDFDDLICMP TLLLKNNKKI KIRWQKKISY LLVDEYQDTN NSQYELIKML ANKNSDFTLV
     GDDDQSIYSW RGANPQNIFL LKKDFPNLKI IKMEHNYRSS GRILKAANSL IANNIHYLEK
     KLFSQLKYGN LIKVLIGKNE ENEAQKIVKK IISQYAKKKI KYRDFAILYR GNYQSRILEK
     ALIKENIPYN ISEKSSFFSR PEIKDLLSYL RVVINRDDNH AFMRIVNIPS RQIGKTTLKK
     LEEWANKKHV SLFQASNNIE IKKFLNENTI KKIKNFISKI EKFTAWSCLK PSNIIDDIVD
     DLEYEKWLSK FLKDPNKIKN SINNVHTLSQ WFKNMIKGDD FEKPMTLFQI VTRMTIRDIL
     DDNIIKEQQD RVQLMTLHAS KGLEFPAVFI IGMCEGILPN QKSIDNDNIE EERRLTYVGI
     TRAKKQLFFT YCYKRTQYGQ ILDMLPSRFL FELPQEDLKW EKKIF