ID   REP_BUCAP               Reviewed;         658 AA.
AC   O51889;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=ATP-dependent DNA helicase Rep {ECO:0000255|HAMAP-Rule:MF_01920};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01920};
GN   Name=rep {ECO:0000255|HAMAP-Rule:MF_01920}; OrderedLocusNames=BUsg_574;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9516544; DOI=10.1007/pl00006760;
RA   Clark M.A., Baumann L., Baumann P.;
RT   "Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera
RT   aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon,
RT   gidA, and rho.";
RL   Curr. Microbiol. 36:158-163(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Rep helicase is a single-stranded DNA-dependent ATPase
CC       involved in DNA replication; it can initiate unwinding at a nick in the
CC       DNA. It binds to the single-stranded DNA and acts in a progressive
CC       fashion along the DNA in the 3' to 5' direction. {ECO:0000255|HAMAP-
CC       Rule:MF_01920}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01920};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01920}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01920}.
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DR   EMBL; AF008210; AAC38127.1; -; Genomic_DNA.
DR   EMBL; AE013218; AAM68108.1; -; Genomic_DNA.
DR   RefSeq; WP_011054074.1; NC_004061.1.
DR   AlphaFoldDB; O51889; -.
DR   SMR; O51889; -.
DR   STRING; 198804.BUsg_574; -.
DR   EnsemblBacteria; AAM68108; AAM68108; BUsg_574.
DR   KEGG; bas:BUsg_574; -.
DR   eggNOG; COG0210; Bacteria.
DR   HOGENOM; CLU_004585_5_2_6; -.
DR   OMA; HCANILI; -.
DR   OrthoDB; 137860at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_01920; Helicase_Rep; 1.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR005752; Helicase_Rep.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01074; rep; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..658
FT                   /note="ATP-dependent DNA helicase Rep"
FT                   /id="PRO_0000102066"
FT   DOMAIN          1..280
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   DOMAIN          281..564
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   BINDING         22..29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT   BINDING         278
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
SQ   SEQUENCE   658 AA;  77509 MW;  C5CE91AC64A73B3B CRC64;
     MSLNFNQKNA IELINGPCLI LAGAGSGKTK VIINKIIYLI NNCQYKPGNI IAVTFTNKAA
     HEIKVRLAKH LNLLQIKKMI ISTFHSLGLE IIKKEINTLK FNSNFSLFDE RDQMMLLKKI
     CSKSIKNDTK LLKKLVFMIS FWKNKFLTPL QVQLSAQSNL EKDFAFFYKQ YTFHLRKSNI
     LDFDDLICIP TSLLKNNQII QNRWQKKISY LLVDEYQDTN NSQYELIKML TNVNSNFTLV
     GDDDQSIYSW RGAKPQNLFL IKKDFPNLKI IKMEQNYRSY GRILKAANKL ISNNLHYFKK
     KLFSNLEYGN KIKVIIGKNE KNEAEKIADK IIHECSNDIM QYKDYAILYR GNYQSQILEK
     TFLKKNIPYD ISTNSSFFSR PEIKDLLSYL RLIVNPDDNH AFIRILNIPH RQIGLTTLNK
     LEELASKKNK SLFQISNDIE IKKILRERTV KKIKDFIYWI KKIIKLSLLK EDIILDKIIN
     DIKYELWLTK ILKEPKKIKT SINNIYTLSN WLKEMLRGNE FEKPMNLLQI VKKMTLRDIL
     EKKIQINEIP KNRVQLMTLH SSKGLEFSSV FIIGMNEGIL PNIKSINNDN IEEERRLTYV
     GMTRARKELF FTYCQTRIQY GQKLYTAPSR FLFELPQEDL QWEKDDYLDA FHTKEKKI