REP_HAEIN
ID REP_HAEIN Reviewed; 670 AA.
AC P44804;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=ATP-dependent DNA helicase Rep {ECO:0000255|HAMAP-Rule:MF_01920};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01920};
GN Name=rep {ECO:0000255|HAMAP-Rule:MF_01920}; OrderedLocusNames=HI_0649;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Rep helicase is a single-stranded DNA-dependent ATPase
CC involved in DNA replication; it can initiate unwinding at a nick in the
CC DNA. It binds to the single-stranded DNA and acts in a progressive
CC fashion along the DNA in the 3' to 5' direction. {ECO:0000255|HAMAP-
CC Rule:MF_01920}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01920};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01920}.
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01920}.
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DR EMBL; L42023; AAC22309.1; -; Genomic_DNA.
DR RefSeq; NP_438809.1; NC_000907.1.
DR RefSeq; WP_010869028.1; NC_000907.1.
DR AlphaFoldDB; P44804; -.
DR SMR; P44804; -.
DR STRING; 71421.HI_0649; -.
DR EnsemblBacteria; AAC22309; AAC22309; HI_0649.
DR KEGG; hin:HI_0649; -.
DR PATRIC; fig|71421.8.peg.678; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_4_6; -.
DR OMA; HCANILI; -.
DR PhylomeDB; P44804; -.
DR BioCyc; HINF71421:G1GJ1-684-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_01920; Helicase_Rep; 1.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR005752; Helicase_Rep.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01074; rep; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..670
FT /note="ATP-dependent DNA helicase Rep"
FT /id="PRO_0000102069"
FT DOMAIN 1..280
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT DOMAIN 281..563
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT BINDING 278
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
SQ SEQUENCE 670 AA; 77746 MW; 802A131251ED4E24 CRC64;
MKLNPQQQQA VEYVTGPCLV LAGAGSGKTR VIINKIAHLI EKCGYSPKQI AAVTFTNKAA
REMKERVAHS IGKEQSKGLL VSTFHTLGFD ILKREYKALG FKSNMTLFDE HDQFALLKEL
TADVLKEDKD LLRELISVIS NWKNDLISPK QAFALARDAK YQTFAKCYER YATQIRTYNA
LDFDDLIMLP TLLFKQNEEV RSKWQAKIRY LLVDEYQDTN TSQYELIKLL VGDRACFTVV
GDDDQSIYSW RGARPENMVR LRDDFPRLNV IKLEQNYRST QRILHCANIL IDNNEHVFDK
KLFSTIGKGE KLLVIEAKNE EHEAERIVAE LIAHRFSRKT KYKDYAILYR GNHQSRLLEK
VLMQNRIPYK ISGGTSFFSR AEIKDMMAYL RLVVNQDDDA AFLRIVNTPK REIGTATLQK
LGELAQEKHI SLFEAIFEFE LIQRITPKAY DSLQKFGRWI VELNDEIQRS EPERAVRSML
SAIHYEEYLY EYATSPKAAE MQSKNVATLF DWVADMLKGD ETNEPMNLNQ VVTRLTLRDM
LERGEDDDDS DQVQLMTLHA SKGLEFPYVY LIGMEEGILP HQTSIDEDNV EEERRLAYVG
ITRAQKELTF SLCRERRQYG ELVRPEPSRF LAELPNDDVL WERDKPKLTT EQKQEKTQNQ
LDRLRAILKS
