ID   REP_LENHI               Reviewed;         314 AA.
AC   P35857;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Protein rep;
DE   AltName: Full=Replication protein;
GN   Name=rep;
OS   Lentilactobacillus hilgardii (Lactobacillus hilgardii).
OG   Plasmid pLAB1000.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lentilactobacillus.
OX   NCBI_TaxID=1588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2188951; DOI=10.1128/jb.172.6.3089-3099.1990;
RA   Josson K., Soetaert P., Michiels F., Joos H., Mahillon J.;
RT   "Lactobacillus hilgardii plasmid pLAB1000 consists of two functional
RT   cassettes commonly found in other Gram-positive organisms.";
RL   J. Bacteriol. 172:3089-3099(1990).
CC   -!- FUNCTION: Produces a single-strand nick in a specific site of the
CC       plasmid, and this nick results in single-strand replication by rolling
CC       circle mechanism.
CC   -!- SIMILARITY: Belongs to the Gram-positive plasmids replication protein
CC       type 1 family. {ECO:0000305}.
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DR   EMBL; M55222; AAA98163.1; -; Genomic_DNA.
DR   PIR; B35390; B35390.
DR   RefSeq; WP_033179285.1; NC_025165.1.
DR   RefSeq; YP_009070088.1; NC_025165.1.
DR   AlphaFoldDB; P35857; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR000989; Rep.
DR   Pfam; PF01446; Rep_1; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Protein rep"
FT                   /id="PRO_0000068320"
FT   BINDING         229
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  37045 MW;  951446121859D8EB CRC64;
     MSKKILKDVS RNRKERPWRE RKLENLQYAE YLRILNFKKA NRVKECGEVL RFVADDEGRL
     RLYQTWFCKS RLCPLCNWRR SMGQSNQLMQ VLDEAHKQRK TGRFLFLTLT AENASGENLK
     QEVRKMGRAI SKLFQYKKPA KNLLGYVRST EITINKNGTY HQHMHVLLFV KPTYFKDSAN
     YINQAEWSKL WQRAMKLDYQ PIVNVEAVRS NKAKGKNSLI ASAQETAKYQ VKSKDILTND
     QERDLQVVED LEQGLAGSRQ ISYGGLFKEI RKQLQLEDVD AHLINVDDDK VKIDEVVREV
     VAKWDYNKQN YFIW