REP_SALTY
ID REP_SALTY Reviewed; 674 AA.
AC Q9L6S1;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=ATP-dependent DNA helicase Rep {ECO:0000255|HAMAP-Rule:MF_01920};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01920};
GN Name=rep {ECO:0000255|HAMAP-Rule:MF_01920}; OrderedLocusNames=STM3912;
GN ORFNames=STMD1.79;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Rep helicase is a single-stranded DNA-dependent ATPase
CC involved in DNA replication; it can initiate unwinding at a nick in the
CC DNA. It binds to the single-stranded DNA and acts in a progressive
CC fashion along the DNA in the 3' to 5' direction. {ECO:0000255|HAMAP-
CC Rule:MF_01920}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01920};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01920}.
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01920}.
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DR EMBL; AF233324; AAF33474.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22762.1; -; Genomic_DNA.
DR RefSeq; NP_462803.1; NC_003197.2.
DR RefSeq; WP_001238842.1; NC_003197.2.
DR AlphaFoldDB; Q9L6S1; -.
DR SMR; Q9L6S1; -.
DR STRING; 99287.STM3912; -.
DR PaxDb; Q9L6S1; -.
DR EnsemblBacteria; AAL22762; AAL22762; STM3912.
DR GeneID; 1255438; -.
DR KEGG; stm:STM3912; -.
DR PATRIC; fig|99287.12.peg.4134; -.
DR HOGENOM; CLU_004585_5_4_6; -.
DR OMA; HCANILI; -.
DR PhylomeDB; Q9L6S1; -.
DR BioCyc; SENT99287:STM3912-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_01920; Helicase_Rep; 1.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR005752; Helicase_Rep.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01074; rep; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..674
FT /note="ATP-dependent DNA helicase Rep"
FT /id="PRO_0000102070"
FT DOMAIN 1..280
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT DOMAIN 281..562
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
FT BINDING 278
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01920"
SQ SEQUENCE 674 AA; 77171 MW; CC561D79E8B5A5D6 CRC64;
MRLNPGQQHA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI AAVTFTNKAA
REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG MKSNFSLFDD TDQVALLKEL
TEGLIEDDKV LLQQLISTIS NWKNDLKTPA QAAAGAKGER DRIFAHCYGL YDAHMKACNV
LDFDDLILLP TLLLQRNDEV RERWQNKIRY LLVDEYQDTN TSQYELVKLL VGQRARFTVV
GDDDQSIYSW RGARPQNLVL LSQDFPALQV IKLEQNYRSS GRILKAANIL IANNPHVFEK
RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR GNHQSRVFEK
FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS AFLRIVNTPK REIGPATLQK
LGEWAMTRNK SLFTASFDMG LSQKLTGRGY DSLTRFTHWL GEIQRLAERE PVAAVRDLIH
GIDYESWLYE TSPSPKAAEM RMKNVNQLFS WMTEMLEGNE LDEPMTLTQV VTRFTLRDMM
ERGESEEELD QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNIE EERRLAYVGI
TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE ERMQKGQSHL
ANLKAMMAAK RAKS
