ID   REP_STAAU               Reviewed;         314 AA.
AC   P08115;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Replication initiation protein;
GN   Name=rep;
OS   Staphylococcus aureus.
OG   Plasmid pTZ12.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3110008; DOI=10.1016/0378-1119(87)90481-1;
RA   Aoki T., Noguchi N., Sasatsu M., Kono M.;
RT   "Complete nucleotide sequence of pTZ12, a chloramphenicol-resistance
RT   plasmid of Bacillus subtilis.";
RL   Gene 51:107-111(1987).
CC   -!- FUNCTION: This protein is probably a specific topoisomerase involved in
CC       initiating replication. This protein is specifically required and may
CC       be rate-limiting for replication of the plasmid in vivo.
CC   -!- MISCELLANEOUS: The nicking site of REP proteins is sequence, but not
CC       plasmid, specific.
CC   -!- MISCELLANEOUS: It appears that the REP protein of pTZ12 is a fusion
CC       protein of pT181 RepC and pC221 RepD proteins, formed spontaneously by
CC       a recombination event between a pT181-like plasmid and a pC221-like
CC       plasmid.
CC   -!- SIMILARITY: Belongs to the plasmid replication initiation factor
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M16192; AAA72571.1; -; Genomic_DNA.
DR   AlphaFoldDB; P08115; -.
DR   SMR; P08115; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   InterPro; IPR003491; Rep_trans.
DR   Pfam; PF02486; Rep_trans; 1.
PE   3: Inferred from homology;
KW   DNA replication; Plasmid.
FT   CHAIN           1..314
FT                   /note="Replication initiation protein"
FT                   /id="PRO_0000068327"
SQ   SEQUENCE   314 AA;  37874 MW;  900144B659C11B62 CRC64;
     MYKNNHANHS NHLENHDLDN FSKTGYSNSR LSGNFFTTPQ PELSFDAMTI VGNLNKTNAK
     KLSDFMSTEP QIRLWDILQT KFKAKALQEK VYIEYDKVKA DSWDRRNMRV EFNPNKLTHE
     EMLWLKQNII DYMEDDGFTR LDLAFDFEDD LSDYYAMTDK AVKKTIFYGR NGKPETKYFG
     VRDSDRFIRI YNKKQERKDN ADVEVMSEHL WRVEIELKRD MVDYWNDCFD DLHILKPDWT
     TPEKVKEQAM VYLLLNEEGT WGKLERHAKY KYKQLIKEIS PIDLTELMKS TLKENEKQLQ
     KQIDFWQREF RFWK