ID   RES4_ECOLX              Reviewed;         190 AA.
AC   P13606;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=R46 site-specific recombinase;
GN   Name=tnpR; Synonyms=perR46;
OS   Escherichia coli.
OG   Plasmid IncN R46.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2832517; DOI=10.1099/00221287-133-8-2031;
RA   Dodd H.M., Bennett P.M.;
RT   "The R46 site-specific recombination system is a homologue of the Tn3 and
RT   gamma delta (Tn1000) cointegrate resolution system.";
RL   J. Gen. Microbiol. 133:2031-2039(1987).
CC   -!- FUNCTION: Site-specific recombination protein.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; M17311; AAA26080.1; -; Genomic_DNA.
DR   AlphaFoldDB; P13606; -.
DR   SMR; P13606; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid.
FT   CHAIN           1..190
FT                   /note="R46 site-specific recombinase"
FT                   /id="PRO_0000196371"
FT   DOMAIN          2..137
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        161..180
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        10
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   190 AA;  21152 MW;  27A6D6D873B23953 CRC64;
     MRLFGYARVS TSQQSLDIQI KGLKEAGVKA SRIFTDKASG SSTDRKGLDL LRMKVEEGDV
     TLVKKLDRLG RDTADMIQLT KEFDAQGVAV RFIDDGISTD GEMGKMVVTI LSAVAQAERR
     RILERTNEGR QEAKLKGIRF GRKRIIDRNS VLALHQQGTG ATDIARRLSI ARSTVYKILE
     DESRVNLSKI