RESIL_DROME
ID RESIL_DROME Reviewed; 620 AA.
AC Q9V7U0; Q86P83;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Pro-resilin;
DE Flags: Precursor;
GN Name=resilin; ORFNames=CG15920;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF57953.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF57953.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAO25082.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=11483432; DOI=10.1016/s0965-1748(01)00044-3;
RA Ardell D.H., Andersen S.O.;
RT "Tentative identification of a resilin gene in Drosophila melanogaster.";
RL Insect Biochem. Mol. Biol. 31:965-970(2001).
RN [5] {ECO:0000305}
RP DEVELOPMENTAL STAGE, AND BIOTECHNOLOGICAL RELEVANCE.
RX PubMed=16222249; DOI=10.1038/nature04085;
RA Elvin C.M., Carr A.G., Huson M.G., Maxwell J.M., Pearson R.D., Vuocolo T.,
RA Liyou N.E., Wong D.C.C., Merritt D.J., Dixon N.E.;
RT "Synthesis and properties of crosslinked recombinant pro-resilin.";
RL Nature 437:999-1002(2005).
CC -!- FUNCTION: Plays a central role in insect flight by providing low
CC stiffness, high strain and efficient energy storage. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000303|PubMed:10731132};
CC IsoId=Q9V7U0-1; Sequence=Displayed;
CC Name=B {ECO:0000303|PubMed:10731132};
CC IsoId=Q9V7U0-2; Sequence=VSP_051872;
CC -!- DEVELOPMENTAL STAGE: Expressed at pupal stage only.
CC {ECO:0000269|PubMed:16222249}.
CC -!- BIOTECHNOLOGY: Its strong elasticity suggests that it may be used for
CC industrial and pharmaceutical applications. Recombinant protein can be
CC cast into a rubber-like biomaterial by rapid photochemical cross-
CC linking. The resilience (recovery after deformation) of cross-linked
CC recombinant resilin exceeds that of unfilled synthetic polybutadiene, a
CC high resilience rubber. {ECO:0000269|PubMed:16222249}.
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DR EMBL; AE013599; AAF57953.1; -; Genomic_DNA.
DR EMBL; AE013599; AAS64829.1; -; Genomic_DNA.
DR EMBL; BT003322; AAO25082.1; -; mRNA.
DR RefSeq; NP_611157.1; NM_137313.4. [Q9V7U0-1]
DR RefSeq; NP_995860.1; NM_206138.2. [Q9V7U0-2]
DR AlphaFoldDB; Q9V7U0; -.
DR BioGRID; 62589; 1.
DR STRING; 7227.FBpp0086175; -.
DR PaxDb; Q9V7U0; -.
DR PRIDE; Q9V7U0; -.
DR EnsemblMetazoa; FBtr0087024; FBpp0086175; FBgn0034157. [Q9V7U0-1]
DR EnsemblMetazoa; FBtr0087025; FBpp0086176; FBgn0034157. [Q9V7U0-2]
DR GeneID; 36880; -.
DR KEGG; dme:Dmel_CG15920; -.
DR UCSC; CG15920-RA; d. melanogaster. [Q9V7U0-1]
DR CTD; 36880; -.
DR FlyBase; FBgn0034157; resilin.
DR VEuPathDB; VectorBase:FBgn0034157; -.
DR eggNOG; ENOG502S3I7; Eukaryota.
DR HOGENOM; CLU_448552_0_0_1; -.
DR InParanoid; Q9V7U0; -.
DR OMA; PGGQDFG; -.
DR PhylomeDB; Q9V7U0; -.
DR BioGRID-ORCS; 36880; 0 hits in 1 CRISPR screen.
DR ChiTaRS; resilin; fly.
DR GenomeRNAi; 36880; -.
DR PRO; PR:Q9V7U0; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034157; Expressed in pupa and 3 other tissues.
DR Genevisible; Q9V7U0; DM.
DR GO; GO:0062129; C:chitin-based extracellular matrix; ISM:FlyBase.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0032992; C:protein-carbohydrate complex; IDA:FlyBase.
DR GO; GO:0008061; F:chitin binding; IDA:FlyBase.
DR GO; GO:0030023; F:extracellular matrix constituent conferring elasticity; IDA:FlyBase.
DR GO; GO:0008010; F:structural constituent of chitin-based larval cuticle; ISM:FlyBase.
DR GO; GO:0042302; F:structural constituent of cuticle; NAS:UniProtKB.
DR GO; GO:0040003; P:chitin-based cuticle development; ISM:FlyBase.
DR InterPro; IPR031311; CHIT_BIND_RR_consensus.
DR InterPro; IPR000618; Insect_cuticle.
DR Pfam; PF00379; Chitin_bind_4; 1.
DR PROSITE; PS00233; CHIT_BIND_RR_1; 1.
DR PROSITE; PS51155; CHIT_BIND_RR_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cuticle; Reference proteome; Repeat; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..620
FT /note="Pro-resilin"
FT /evidence="ECO:0000255"
FT /id="PRO_0000042845"
FT REPEAT 42..56
FT /note="Elastic 1"
FT /evidence="ECO:0000255"
FT REPEAT 57..77
FT /note="Elastic 2"
FT /evidence="ECO:0000255"
FT REPEAT 78..97
FT /note="Elastic 3"
FT /evidence="ECO:0000255"
FT REPEAT 98..112
FT /note="Elastic 4"
FT /evidence="ECO:0000255"
FT REPEAT 113..127
FT /note="Elastic 5"
FT /evidence="ECO:0000255"
FT REPEAT 128..145
FT /note="Elastic 6"
FT /evidence="ECO:0000255"
FT REPEAT 146..164
FT /note="Elastic 7"
FT /evidence="ECO:0000255"
FT REPEAT 165..179
FT /note="Elastic 8"
FT /evidence="ECO:0000255"
FT REPEAT 180..194
FT /note="Elastic 9"
FT /evidence="ECO:0000255"
FT REPEAT 195..210
FT /note="Elastic 10"
FT /evidence="ECO:0000255"
FT REPEAT 211..225
FT /note="Elastic 11"
FT /evidence="ECO:0000255"
FT REPEAT 226..244
FT /note="Elastic 12"
FT /evidence="ECO:0000255"
FT REPEAT 245..262
FT /note="Elastic 13"
FT /evidence="ECO:0000255"
FT REPEAT 263..276
FT /note="Elastic 14"
FT /evidence="ECO:0000255"
FT REPEAT 277..292
FT /note="Elastic 15"
FT /evidence="ECO:0000255"
FT REPEAT 293..307
FT /note="Elastic 16"
FT /evidence="ECO:0000255"
FT REPEAT 308..324
FT /note="Elastic 17"
FT /evidence="ECO:0000255"
FT DOMAIN 342..415
FT /note="Chitin-binding type R&R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT REGION 22..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..299
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..596
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 341..385
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132, ECO:0000303|Ref.3"
FT /id="VSP_051872"
SQ SEQUENCE 620 AA; 58604 MW; 432AFA06E54CB6BA CRC64;
MFKLLGLTLL MAMVVLGRPE PPVNSYLPPS DSYGAPGQSG PGGRPSDSYG APGGGNGGRP
SDSYGAPGQG QGQGQGQGGY AGKPSDTYGA PGGGNGNGGR PSSSYGAPGG GNGGRPSDTY
GAPGGGNGGR PSDTYGAPGG GGNGNGGRPS SSYGAPGQGQ GNGNGGRSSS SYGAPGGGNG
GRPSDTYGAP GGGNGGRPSD TYGAPGGGNN GGRPSSSYGA PGGGNGGRPS DTYGAPGGGN
GNGSGGRPSS SYGAPGQGQG GFGGRPSDSY GAPGQNQKPS DSYGAPGSGN GNGGRPSSSY
GAPGSGPGGR PSDSYGPPAS GSGAGGAGGS GPGGADYDND EPAKYEFNYQ VEDAPSGLSF
GHSEMRDGDF TTGQYNVLLP DGRKQIVEYE ADQQGYRPQI RYEGDANDGS GPSGPGGPGG
QNLGADGYSS GRPGNGNGNG NGGYSGGRPG GQDLGPSGYS GGRPGGQDLG AGGYSNGKPG
GQDLGPGGYS GGRPGGQDLG RDGYSGGRPG GQDLGASGYS NGRPGGNGNG GSDGGRVIIG
GRVIGGQDGG DQGYSGGRPG GQDLGRDGYS SGRPGGRPGG NGQDSQDGQG YSSGRPGQGG
RNGFGPGGQN GDNDGSGYRY
