ID   RES_PHOLL               Reviewed;         156 AA.
AC   Q7N4H9;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Toxin Res {ECO:0000305|PubMed:30315706};
DE            EC=2.4.2.- {ECO:0000250|UniProtKB:P9WLP9};
GN   Name=res {ECO:0000303|PubMed:30315706}; OrderedLocusNames=plu2358;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS   TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA   Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA   Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
RN   [2]
RP   FUNCTION AS A TOXIN, EXPRESSION IN E.COLI, AND MUTAGENESIS OF ARG-27;
RP   TYR-37; GLU-48 AND SER-115.
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=30315706; DOI=10.1111/mmi.14150;
RA   Skjerning R.B., Senissar M., Winther K.S., Gerdes K., Brodersen D.E.;
RT   "The RES domain toxins of RES-Xre toxin-antitoxin modules induce cell
RT   stasis by degrading NAD+.";
RL   Mol. Microbiol. 111:221-236(2019).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Expression in E.coli inhibits cell growth; bacteriostasis is
CC       neutralized by expression of cognate antitoxin Xre. Expression in
CC       E.coli leads to almost complete depletion of intracellular NAD(+):
CC       NAD(+) levels are partially restored when coexpressed with antitoxin
CC       Xre. {ECO:0000269|PubMed:30315706}.
CC   -!- SUBUNIT: Homodimer. Forms a complex with cognate antitoxin Xre.
CC       {ECO:0000250|UniProtKB:Q88K57}.
CC   -!- SIMILARITY: Belongs to the MbcT/ParT/Res family. {ECO:0000305}.
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DR   EMBL; BX571866; CAE14651.1; -; Genomic_DNA.
DR   RefSeq; WP_011146594.1; NC_005126.1.
DR   AlphaFoldDB; Q7N4H9; -.
DR   SMR; Q7N4H9; -.
DR   STRING; 243265.plu2358; -.
DR   EnsemblBacteria; CAE14651; CAE14651; plu2358.
DR   GeneID; 24166292; -.
DR   KEGG; plu:plu2358; -.
DR   eggNOG; COG5654; Bacteria.
DR   HOGENOM; CLU_133611_0_1_6; -.
DR   OMA; VTYCATS; -.
DR   OrthoDB; 1821714at2; -.
DR   BioCyc; PLUM243265:PLU_RS11720-MON; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR014914; RES_dom.
DR   Pfam; PF08808; RES; 1.
DR   SMART; SM00953; RES; 1.
PE   1: Evidence at protein level;
KW   NAD; Nucleotidyltransferase; Reference proteome; Toxin-antitoxin system;
KW   Transferase.
FT   CHAIN           1..156
FT                   /note="Toxin Res"
FT                   /id="PRO_0000448606"
FT   MUTAGEN         27
FT                   /note="R->A: No longer toxic in E.coli, E.coli
FT                   intracellular NAD(+) levels do not change."
FT                   /evidence="ECO:0000269|PubMed:30315706"
FT   MUTAGEN         37
FT                   /note="Y->A: Significantly decreased toxicity in E.coli, in
FT                   liquid culture is not neutralized by antitoxin Xre,
FT                   partially neutralized when grown on solid medium."
FT                   /evidence="ECO:0000269|PubMed:30315706"
FT   MUTAGEN         48
FT                   /note="E->A: No longer toxic in E.coli."
FT                   /evidence="ECO:0000269|PubMed:30315706"
FT   MUTAGEN         115
FT                   /note="S->A: No longer toxic in E.coli."
FT                   /evidence="ECO:0000269|PubMed:30315706"
SQ   SEQUENCE   156 AA;  17374 MW;  BB8993968B2E46B0 CRC64;
     MILYRLTRSK YVESAWSGTG AKLYGGRWHN IGRPAVYVAT SVSLAVLEVL VHVGDDELLT
     DFALLSIDIP ENQIDILDID TLPSDWNAPV PSTCTMEIGS EWFEVSHSIG LVVPSAIVPY
     ENNVILNPMA KDFHKYINTV KRLDFGIDSR LVKAKK