RETM_DROME
ID RETM_DROME Reviewed; 659 AA.
AC Q9VMD6; Q95U56;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein real-time;
GN Name=retm; ORFNames=CG9528;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND RNA EDITING OF POSITION 245.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15700158; DOI=10.1007/s00427-005-0470-4;
RA Dee C.T., Moffat K.G.;
RT "A novel family of mitochondrial proteins is represented by the Drosophila
RT genes slmo, preli-like and real-time.";
RL Dev. Genes Evol. 215:248-254(2005).
RN [5]
RP RNA EDITING OF POSITION 245.
RX PubMed=17018572; DOI=10.1261/rna.254306;
RA Stapleton M., Carlson J.W., Celniker S.E.;
RT "RNA editing in Drosophila melanogaster: new targets and functional
RT consequences.";
RL RNA 12:1922-1932(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-477, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:15700158}.
CC -!- TISSUE SPECIFICITY: Restricted to the developing gut and central
CC nervous system (CNS). {ECO:0000269|PubMed:15700158}.
CC -!- DEVELOPMENTAL STAGE: Detected from at stage 7, where it is expressed in
CC the early germ band. By stage 11, when the germ band is fully extended,
CC it is expressed strongly in the ventral neuroectoderm, which represents
CC the progenitor of the CNS. At this time, a small region of particularly
CC strong expression is visible at the tip of the extended germ band
CC marking the proctodeum (the primordial hindgut) and expression is also
CC visible in the anterior midgut and the foregut primordial region known
CC as the stomodeum. Expression then persists in both the emerging gut and
CC nervous system during the next three developmental stages (12-14).
CC Strong expression in the primordial midgut is rapidly down-regulated
CC prior to stage 15, by when the presumptive midgut has closed both
CC ventrally and dorsally and is largely established. However, expression
CC persists in the hindgut as it continues to develop by projecting
CC towards the antero-dorsal region of the embryo and is also evident in
CC differentiating components of the foregut, which might include the
CC stomatogastric nervous system. Interestingly, it is expressed strongly
CC throughout the developing CNS and the intensity of this staining is
CC maintained through stage 16 during major periods of developmental
CC reorganization, while expression in regions of the gut is rapidly down-
CC regulated. Expression continues in the CNS late into stage 17, but is
CC also clearly reduced by the end of stage 17 immediately prior to
CC hatching. Expressed during all subsequent developmental stages from
CC first instar larvae through to adulthood.
CC {ECO:0000269|PubMed:15700158}.
CC -!- RNA EDITING: Modified_positions=245 {ECO:0000269|PubMed:12537569,
CC ECO:0000269|PubMed:17018572}; Note=Partially edited. Target of Adar.;
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DR EMBL; AE014134; AAF52383.2; -; Genomic_DNA.
DR EMBL; AY058298; AAL13527.1; -; mRNA.
DR RefSeq; NP_001285672.1; NM_001298743.1.
DR RefSeq; NP_609028.2; NM_135184.4.
DR AlphaFoldDB; Q9VMD6; -.
DR SMR; Q9VMD6; -.
DR BioGRID; 60056; 1.
DR STRING; 7227.FBpp0078902; -.
DR iPTMnet; Q9VMD6; -.
DR PaxDb; Q9VMD6; -.
DR DNASU; 33899; -.
DR EnsemblMetazoa; FBtr0079272; FBpp0078902; FBgn0031814.
DR EnsemblMetazoa; FBtr0339788; FBpp0308835; FBgn0031814.
DR GeneID; 33899; -.
DR KEGG; dme:Dmel_CG9528; -.
DR UCSC; CG9528-RA; d. melanogaster.
DR CTD; 33899; -.
DR FlyBase; FBgn0031814; retm.
DR VEuPathDB; VectorBase:FBgn0031814; -.
DR eggNOG; KOG1471; Eukaryota.
DR GeneTree; ENSGT00940000168605; -.
DR HOGENOM; CLU_023840_0_0_1; -.
DR InParanoid; Q9VMD6; -.
DR OMA; NAEWTCF; -.
DR OrthoDB; 1133487at2759; -.
DR PhylomeDB; Q9VMD6; -.
DR BioGRID-ORCS; 33899; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33899; -.
DR PRO; PR:Q9VMD6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031814; Expressed in mouthpart and 21 other tissues.
DR ExpressionAtlas; Q9VMD6; baseline and differential.
DR Genevisible; Q9VMD6; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR CDD; cd00170; SEC14; 1.
DR Gene3D; 3.40.525.10; -; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR011074; CRAL/TRIO_N_dom.
DR InterPro; IPR036273; CRAL/TRIO_N_dom_sf.
DR InterPro; IPR009038; GOLD_dom.
DR InterPro; IPR036598; GOLD_dom_sf.
DR InterPro; IPR006797; PRELI/MSF1_dom.
DR Pfam; PF00650; CRAL_TRIO; 1.
DR Pfam; PF03765; CRAL_TRIO_N; 1.
DR Pfam; PF04707; PRELI; 1.
DR SMART; SM01100; CRAL_TRIO_N; 1.
DR SMART; SM00516; SEC14; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR SUPFAM; SSF46938; SSF46938; 1.
DR SUPFAM; SSF52087; SSF52087; 1.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS50866; GOLD; 1.
DR PROSITE; PS50904; PRELI_MSF1; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Phosphoprotein; Reference proteome; RNA editing.
FT CHAIN 1..659
FT /note="Protein real-time"
FT /id="PRO_0000210763"
FT DOMAIN 3..175
FT /note="PRELI/MSF1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00158"
FT DOMAIN 286..462
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00056"
FT DOMAIN 512..631
FT /note="GOLD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT MOD_RES 477
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VARIANT 245
FT /note="Q -> R (in RNA edited version)"
SQ SEQUENCE 659 AA; 75679 MW; 3E6D07E266081080 CRC64;
MVQKFQSPVR VYKYPFELVM KAYERRFPTC PQMPIVLDCE VIKDESLEDG AKRNTSRRCK
LAVDAPYIFK KLIGVDHVYF LQHNFLDLAN RTLSIEAVNE SFSSRIEIFE RCRYYAHPDN
SEWTCFDQSA TLDIKNFFGF EHSMEKMGMK QYTQTTLKGK EIIEFFIGQL REEGITHVER
WTSPSDATKS PTLDQASDQQ HSILLDGDFI ARSLGQLSPM QESKLLELRK MLDGVDDLER
VPSYQTILRF LAARDWHVSQ AYAMLCDSLR WRREHRIDAL LAEYSKPAVV VEHFPGGWHH
LDKDGRPVYI LRLGHMDVKG LLKSLGMDGL LRLALHICEE GIQKINESAE RLEKPVLNWS
LLVDLEGLSM RHLWRPGIKA LLNIIETVER NYPETMGRVL VVRAPRVFPI AWTIVSAFID
EHTRSKFLFY GPDCAHMKDG LAQYLDEEIV PDFLGGPCKT MIHEGGLVPK TLYKMNSLED
HDDEVTAELP TTAAAQALVP GKRLSANQQH DHRNLYKSVD LKAGFAHELL IRNEDPKSVL
TWDFDVMRND LHFTLYRVTQ ELPEKNDSAV SYFDLQDFVE GVNYFREEPT LICRHKESVQ
GSHVMHHNDS YLMHWFSPSG AQLNVFYEVL SSANYKGSMT SLQSAFSSNS SAASSVQSR
