RETR1_BOVIN
ID RETR1_BOVIN Reviewed; 493 AA.
AC Q5E9K8; A5PJF3;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Reticulophagy regulator 1;
DE AltName: Full=Reticulophagy receptor 1 {ECO:0000305};
GN Name=RETREG1; Synonyms=FAM134B;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Crossbred X Angus, and Hereford; TISSUE=Hypothalamus, and Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Endoplasmic reticulum-anchored autophagy receptor that
CC mediates ER delivery into lysosomes through sequestration into
CC autophagosomes. Promotes membrane remodeling and ER scission via its
CC membrane bending capacity and targets the fragments into autophagosomes
CC via interaction with ATG8 family proteins. Required for long-term
CC survival of nociceptive and autonomic ganglion neurons.
CC {ECO:0000250|UniProtKB:Q9H6L5}.
CC -!- SUBUNIT: Interacts with ATG8 family modifier proteins MAP1LC3A,
CC MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2.
CC {ECO:0000250|UniProtKB:Q9H6L5}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000250|UniProtKB:Q8VE91}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9H6L5}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5E9K8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5E9K8-2; Sequence=VSP_057835;
CC -!- DOMAIN: The LIR motif interacts with ATG8 family proteins and is
CC necessary to target the ER fragments to autophagosomes for lysosomal
CC degradation. {ECO:0000250|UniProtKB:Q9H6L5}.
CC -!- DOMAIN: The reticulon homology domain provides capacity to bend the
CC membrane and promotes ER scission (By similarity). This domain does not
CC show relevant similarities with reticulon domains, preventing any
CC domain predictions within the protein sequence.
CC {ECO:0000250|UniProtKB:Q9H6L5, ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RETREG family. {ECO:0000305}.
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DR EMBL; BT020912; AAX08929.1; -; mRNA.
DR EMBL; BC102079; AAI02080.1; -; mRNA.
DR EMBL; BC142089; AAI42090.1; -; mRNA.
DR RefSeq; NP_001015672.1; NM_001015672.2. [Q5E9K8-2]
DR AlphaFoldDB; Q5E9K8; -.
DR STRING; 9913.ENSBTAP00000021862; -.
DR PaxDb; Q5E9K8; -.
DR Ensembl; ENSBTAT00000021862; ENSBTAP00000021862; ENSBTAG00000016444. [Q5E9K8-2]
DR GeneID; 540068; -.
DR KEGG; bta:540068; -.
DR CTD; 54463; -.
DR VEuPathDB; HostDB:ENSBTAG00000016444; -.
DR eggNOG; ENOG502QPW8; Eukaryota.
DR GeneTree; ENSGT00940000160746; -.
DR InParanoid; Q5E9K8; -.
DR OrthoDB; 901531at2759; -.
DR TreeFam; TF329111; -.
DR Proteomes; UP000009136; Chromosome 20.
DR Bgee; ENSBTAG00000016444; Expressed in gluteal muscle and 108 other tissues.
DR ExpressionAtlas; Q5E9K8; baseline and differential.
DR GO; GO:0005801; C:cis-Golgi network; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:GO_Central.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:GO_Central.
DR GO; GO:0061709; P:reticulophagy; ISS:GO_Central.
DR GO; GO:0019233; P:sensory perception of pain; ISS:UniProtKB.
DR InterPro; IPR033358; RETREG1.
DR InterPro; IPR043384; RETREG1/3.
DR PANTHER; PTHR28659; PTHR28659; 1.
DR PANTHER; PTHR28659:SF3; PTHR28659:SF3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Autophagy; Endoplasmic reticulum; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..493
FT /note="Reticulophagy regulator 1"
FT /id="PRO_0000288465"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9H6L5"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..87
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..204
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..493
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9H6L5"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 80..229
FT /note="Reticulon homology domain"
FT /evidence="ECO:0000250|UniProtKB:Q9H6L5"
FT REGION 315..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 435..493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 449..454
FT /note="LIR motif"
FT /evidence="ECO:0000250|UniProtKB:Q9H6L5"
FT COMPBIAS 19..38
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 363..384
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..459
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..486
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 353
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8VE91"
FT VAR_SEQ 1..148
FT /note="MASPAPPEPAEQGSPALAAAPQAPPPPTRAPPEEPEGAAPPEEGAAAGAGRQ
FT VEEAAGGVAAVVTWLLGEPALWLGGRADELLSWKRPLHSLLAFVGANLVFWFLALTPWR
FT VYHLISVMILTRVIMQIIKDMILSRTRGAQLWRSLSE -> MPEGEDFGPGK (in
FT isoform 2)"
FT /id="VSP_057835"
SQ SEQUENCE 493 AA; 53680 MW; 3FD62FAF32342A05 CRC64;
MASPAPPEPA EQGSPALAAA PQAPPPPTRA PPEEPEGAAP PEEGAAAGAG RQVEEAAGGV
AAVVTWLLGE PALWLGGRAD ELLSWKRPLH SLLAFVGANL VFWFLALTPW RVYHLISVMI
LTRVIMQIIK DMILSRTRGA QLWRSLSESW EVINSKPDER PGFSHCLAES WMNFSIFLQE
MSVFKQQSPG KFCLLVCSVC TFFTILGSYI PGVILSYLLL LCAFLCPLFK CNDIGQKIYS
KIKSCLLKLD FGIREYINQK KRERSEADKE KSHKDDSELD FSALCPKISL TTAAKELSVS
DTDVSEVSWT DNGTFNLSEG YTPQTDTSDD LDRPSEEVFS RDLSDFPSLE NGTGTNDEDE
LSLGLPTELK RKKEQLDGGP RRSTEKKSAA GLSLPLSSDQ TLHLMSDLAG DVITAAVTAA
VKDQLAGVRQ ALSQAAPSLG EDTDTEEGDD FELLDQSELD QIESELGLSQ DQEAEAQQNK
KSSGFLSNLL GGH
