RETR3_MOUSE
ID RETR3_MOUSE Reviewed; 466 AA.
AC Q9CQV4; Q3TQL8; Q3U9Q7; Q8BQC4; Q922X3;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Reticulophagy regulator 3;
GN Name=Retreg3; Synonyms=Fam134c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Corpora quadrigemina, and Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-283; SER-285; SER-288;
RP SER-303; THR-310; SER-313 AND SER-320, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=19838196; DOI=10.1038/ng.464;
RA Kurth I., Pamminger T., Hennings J.C., Soehendra D., Huebner A.K.,
RA Rotthier A., Baets J., Senderek J., Topaloglu H., Farrell S.A.,
RA Nuernberg G., Nurnberg P., De Jonghe P., Gal A., Kaether C., Timmerman V.,
RA Huebner C.A.;
RT "Mutations in FAM134B, encoding a newly identified Golgi protein, cause
RT severe sensory and autonomic neuropathy.";
RL Nat. Genet. 41:1179-1181(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; THR-254; SER-258;
RP SER-260; THR-283; SER-285; SER-288; SER-293; THR-307; THR-310; SER-313 AND
RP SER-320, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mediates NRF1-enhanced neurite outgrowth.
CC {ECO:0000250|UniProtKB:Q86VR2}.
CC -!- SUBUNIT: Interacts with ATG8 family modifier proteins MAP1LC3A,
CC MAP1LC3B, GABARAP and GABARAPL1. {ECO:0000250|UniProtKB:Q86VR2}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CQV4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CQV4-2; Sequence=VSP_025689;
CC -!- TISSUE SPECIFICITY: Mainly expressed in the central nervous system and
CC in parenchymatous organs including liver, lung and kidney.
CC {ECO:0000269|PubMed:19838196}.
CC -!- DOMAIN: The LIR motif interacts with ATG8 family proteins.
CC {ECO:0000250|UniProtKB:Q86VR2}.
CC -!- SIMILARITY: Belongs to the RETREG family. {ECO:0000305}.
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DR EMBL; AK004960; BAB23700.1; -; mRNA.
DR EMBL; AK004979; BAB23715.1; -; mRNA.
DR EMBL; AK051025; BAC34501.1; -; mRNA.
DR EMBL; AK151685; BAE30609.1; -; mRNA.
DR EMBL; AK160000; BAE35548.1; -; mRNA.
DR EMBL; AK163482; BAE37364.1; -; mRNA.
DR EMBL; BX255926; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC006712; AAH06712.1; -; mRNA.
DR EMBL; BC016089; AAH16089.1; -; mRNA.
DR CCDS; CCDS25449.1; -. [Q9CQV4-1]
DR CCDS; CCDS25450.1; -. [Q9CQV4-2]
DR RefSeq; NP_080777.1; NM_026501.2. [Q9CQV4-1]
DR RefSeq; NP_083209.1; NM_028933.3. [Q9CQV4-2]
DR AlphaFoldDB; Q9CQV4; -.
DR BioGRID; 212590; 1.
DR STRING; 10090.ENSMUSP00000017946; -.
DR iPTMnet; Q9CQV4; -.
DR PhosphoSitePlus; Q9CQV4; -.
DR EPD; Q9CQV4; -.
DR jPOST; Q9CQV4; -.
DR MaxQB; Q9CQV4; -.
DR PaxDb; Q9CQV4; -.
DR PeptideAtlas; Q9CQV4; -.
DR PRIDE; Q9CQV4; -.
DR ProteomicsDB; 253115; -. [Q9CQV4-1]
DR ProteomicsDB; 253116; -. [Q9CQV4-2]
DR Antibodypedia; 3087; 71 antibodies from 17 providers.
DR Ensembl; ENSMUST00000017946; ENSMUSP00000017946; ENSMUSG00000017802. [Q9CQV4-1]
DR Ensembl; ENSMUST00000107295; ENSMUSP00000102916; ENSMUSG00000017802. [Q9CQV4-2]
DR GeneID; 67998; -.
DR KEGG; mmu:67998; -.
DR UCSC; uc007lni.1; mouse. [Q9CQV4-1]
DR CTD; 162427; -.
DR MGI; MGI:1915248; Retreg3.
DR VEuPathDB; HostDB:ENSMUSG00000017802; -.
DR eggNOG; ENOG502QPTN; Eukaryota.
DR GeneTree; ENSGT00940000161349; -.
DR HOGENOM; CLU_036265_1_0_1; -.
DR InParanoid; Q9CQV4; -.
DR OMA; HIAEAWV; -.
DR OrthoDB; 901531at2759; -.
DR PhylomeDB; Q9CQV4; -.
DR TreeFam; TF329111; -.
DR BioGRID-ORCS; 67998; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Fam134c; mouse.
DR PRO; PR:Q9CQV4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9CQV4; protein.
DR Bgee; ENSMUSG00000017802; Expressed in lip and 244 other tissues.
DR Genevisible; Q9CQV4; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0140506; F:endoplasmic reticulum-autophagosome adaptor activity; IMP:MGI.
DR GO; GO:0030574; P:collagen catabolic process; IMP:MGI.
DR GO; GO:0007029; P:endoplasmic reticulum organization; IMP:MGI.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISS:UniProtKB.
DR GO; GO:0061709; P:reticulophagy; IDA:MGI.
DR InterPro; IPR043384; RETREG1/3.
DR InterPro; IPR033361; RETREG3.
DR PANTHER; PTHR28659; PTHR28659; 1.
DR PANTHER; PTHR28659:SF1; PTHR28659:SF1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..466
FT /note="Reticulophagy regulator 3"
FT /id="PRO_0000288470"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 244..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 285..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 445..450
FT /note="LIR motif"
FT /evidence="ECO:0000250|UniProtKB:Q9H6L5"
FT COMPBIAS 244..262
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 292..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..328
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 254
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 260
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 283
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 285
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 288
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 293
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT MOD_RES 307
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 310
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 313
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 320
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 360
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VR2"
FT MOD_RES 440
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86VR2"
FT VAR_SEQ 1..181
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025689"
FT CONFLICT 340
FT /note="P -> H (in Ref. 1; BAE37364)"
FT /evidence="ECO:0000305"
FT CONFLICT 360
FT /note="S -> T (in Ref. 1; BAE30609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 466 AA; 51638 MW; 5333A7910F023A6C CRC64;
MEEAEGVAAA PGPASGLAFR GRRAMSGSWE RDQQVEAAQR TLVEVLGPYE PLLSRVQAAL
VWERPARSAL WCLGLNAAFW FFALTSLRFV FLLAFSLMII VCIDQWKNKI WPEINVPRPD
ALDNESWGFV HPRLLSVPEL CHHVAEVWVS GTIFIRNLLL FKKQNPGKFC LLSCGVLTFL
AMLGRYIPGL LLSYLMLVII MMWPLAVYHR LWDRAYVRLK PVLQRLDFSV RGYMMSKQRE
RQLRRRALHS ERATDSHSDS EEELAAFCPQ LDDSTVAREL AITDSEHSDA EVSCTENGTF
NLSRGQTPLT EGSEDLDGHS DPEESFARDL PDFPSINVDP AGLDDEDDTS IGMPSLMYRS
PPGAGDTQVL PASRNEAALP ELLLSSLPGG SNLTSNLASL VSQGMIQLAL SEASQTDPSG
PPPRRATRGF LRAPSSDLDT DAEGDDFELL DQSELNQLDP ASSRSH
