REV_ARATH
ID REV_ARATH Reviewed; 842 AA.
AC Q9SE43; A5YZA2; A5YZA3; A5YZA4; A5YZA7; A5YZC3; Q9M5B6;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Homeobox-leucine zipper protein REVOLUTA {ECO:0000303|PubMed:7555701};
DE AltName: Full=HD-ZIP protein REV {ECO:0000303|PubMed:7555701};
DE AltName: Full=Homeodomain transcription factor REV {ECO:0000303|PubMed:7555701};
DE AltName: Full=Protein AMPHIVASAL VASCULAR BUNDLE 1 {ECO:0000303|PubMed:15111711};
DE AltName: Full=Protein INTERFASCICULAR FIBERLESS 1 {ECO:0000303|PubMed:10559440};
GN Name=REV {ECO:0000303|PubMed:7555701};
GN Synonyms=AVB1 {ECO:0000303|PubMed:15111711},
GN IFL1 {ECO:0000303|PubMed:10559440};
GN OrderedLocusNames=At5g60690 {ECO:0000312|Araport:AT5G60690};
GN ORFNames=MUP24.16 {ECO:0000312|EMBL:BAB09842.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10559440; DOI=10.2307/3871015;
RA Zhong R., Ye Z.-H.;
RT "IFL1, a gene regulating interfascicular fiber differentiation in
RT Arabidopsis, encodes a homeodomain-leucine zipper protein.";
RL Plant Cell 11:2139-2152(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10715319; DOI=10.2307/3870938;
RA Ratcliffe O.J., Riechmann J.L., Zhang J.Z.;
RT "INTERFASCICULAR FIBERLESS1 is the same gene as REVOLUTA.";
RL Plant Cell 12:315-317(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 498-653, AND VARIANTS 624-THR-SER-625;
RP SER-625; 625-SER--ILE-627 AND LEU-630.
RC STRAIN=cv. Ag-0, cv. An-1, cv. Br-0, cv. C24, cv. Ct-1, cv. Edi-0,
RC cv. Ga-0, cv. Kas-1, cv. Kin-0, cv. Landsberg erecta, cv. Ll-0, cv. Lz-0,
RC cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Se-0, cv. Sorbo,
RC cv. Tsu-1, cv. Van-0, cv. Wa-1, and cv. Wassilewskija;
RX PubMed=17435248; DOI=10.1534/genetics.107.071928;
RA Ehrenreich I.M., Stafford P.A., Purugganan M.D.;
RT "The genetic architecture of shoot branching in Arabidopsis thaliana: a
RT comparative assessment of candidate gene associations vs. quantitative
RT trait locus mapping.";
RL Genetics 176:1223-1236(2007).
RN [7]
RP FUNCTION.
RX PubMed=7555701; DOI=10.1242/dev.121.9.2723;
RA Talbert P.B., Adler H.T., Parks D.W., Comai L.;
RT "The REVOLUTA gene is necessary for apical meristem development and for
RT limiting cell divisions in the leaves and stems of Arabidopsis thaliana.";
RL Development 121:2723-2735(1995).
RN [8]
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTANT REVOLUTA.
RX PubMed=11169198; DOI=10.1046/j.1365-313x.2001.00959.x;
RA Otsuga D., DeGuzman B., Prigge M.J., Drews G.N., Clark S.E.;
RT "REVOLUTA regulates meristem initiation at lateral positions.";
RL Plant J. 25:223-236(2001).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11402186; DOI=10.1104/pp.126.2.549;
RA Zhong R., Ye Z.-H.;
RT "Alteration of auxin polar transport in the Arabidopsis ifl1 mutants.";
RL Plant Physiol. 126:549-563(2001).
RN [10]
RP FUNCTION, INDUCTION, AND MUTAGENESIS OF PRO-190.
RX PubMed=15111711; DOI=10.1093/pcp/pch051;
RA Zhong R., Ye Z.-H.;
RT "Amphivasal vascular bundle 1, a gain-of-function mutation of the IFL1/REV
RT gene, is associated with alterations in the polarity of leaves, stems and
RT carpels.";
RL Plant Cell Physiol. 45:369-385(2004).
RN [11]
RP FUNCTION.
RX PubMed=15598805; DOI=10.1105/tpc.104.026161;
RA Prigge M.J., Otsuga D., Alonso J.M., Ecker J.R., Drews G.N., Clark S.E.;
RT "Class III homeodomain-leucine zipper gene family members have overlapping,
RT antagonistic, and distinct roles in Arabidopsis development.";
RL Plant Cell 17:61-76(2005).
RN [12]
RP GENE FAMILY.
RX PubMed=16805899; DOI=10.1111/j.1525-142x.2006.00107.x;
RA Prigge M.J., Clark S.E.;
RT "Evolution of the class III HD-Zip gene family in land plants.";
RL Evol. Dev. 8:350-361(2006).
RN [13]
RP INTERACTION WITH ESR1 AND ESR2.
RX PubMed=17376809; DOI=10.1242/dev.001016;
RA Chandler J.W., Cole M., Flier A., Grewe B., Werr W.;
RT "The AP2 transcription factors DORNROSCHEN and DORNROSCHEN-LIKE redundantly
RT control Arabidopsis embryo patterning via interaction with PHAVOLUTA.";
RL Development 134:1653-1662(2007).
RN [14]
RP INDUCTION.
RX PubMed=17237362; DOI=10.1093/pcp/pcm008;
RA Zhou G.-K., Kubo M., Zhong R., Demura T., Ye Z.-H.;
RT "Overexpression of miR165 affects apical meristem formation, organ polarity
RT establishment and vascular development in Arabidopsis.";
RL Plant Cell Physiol. 48:391-404(2007).
RN [15]
RP INTERACTION WITH ZPR1; ZPR2; ZPR3 AND ZPR4.
RX PubMed=18055602; DOI=10.1105/tpc.107.055772;
RA Wenkel S., Emery J., Hou B.H., Evans M.M., Barton M.K.;
RT "A feedback regulatory module formed by LITTLE ZIPPER and HD-ZIPIII
RT genes.";
RL Plant Cell 19:3379-3390(2007).
RN [16]
RP INTERACTION WITH ZPR3.
RX PubMed=18408069; DOI=10.1105/tpc.107.057448;
RA Kim Y.S., Kim S.G., Lee M., Lee I., Park H.Y., Seo P.J., Jung J.H.,
RA Kwon E.J., Suh S.W., Paek K.H., Park C.M.;
RT "HD-ZIP III activity is modulated by competitive inhibitors via a feedback
RT loop in Arabidopsis shoot apical meristem development.";
RL Plant Cell 20:920-933(2008).
RN [17]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY DOF5.1.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=20807212; DOI=10.1111/j.1365-313x.2010.04346.x;
RA Kim H.-S., Kim S.J., Abbasi N., Bressan R.A., Yun D.-J., Yoo S.-D.,
RA Kwon S.-Y., Choi S.-B.;
RT "The DOF transcription factor Dof5.1 influences leaf axial patterning by
RT promoting Revoluta transcription in Arabidopsis.";
RL Plant J. 64:524-535(2010).
RN [18]
RP FUNCTION.
RX PubMed=22781836; DOI=10.1016/j.mod.2012.06.007;
RA Brandt R., Xie Y., Musielak T., Graeff M., Stierhof Y.D., Huang H.,
RA Liu C.M., Wenkel S.;
RT "Control of stem cell homeostasis via interlocking microRNA and
RT microProtein feedback loops.";
RL Mech. Dev. 130:25-33(2013).
RN [19]
RP GENE FAMILY.
RX PubMed=25263420; DOI=10.1016/j.ympev.2014.06.017;
RA Floyd S.K., Ryan J.G., Conway S.J., Brenner E., Burris K.P., Burris J.N.,
RA Chen T., Edger P.P., Graham S.W., Leebens-Mack J.H., Pires J.C.,
RA Rothfels C.J., Sigel E.M., Stevenson D.W., Neal Stewart C. Jr., Wong G.K.,
RA Bowman J.L.;
RT "Origin of a novel regulatory module by duplication and degeneration of an
RT ancient plant transcription factor.";
RL Mol. Phylogenet. Evol. 81:159-173(2014).
CC -!- FUNCTION: Probable transcription factor involved in the regulation of
CC interfascicular fiber (cortical cells) and secondary xylem
CC differentiation in the inflorescence stems. Required for lateral shoot
CC meristems (LSMs) and flower meristems (FMs) initiation. May be involved
CC in the determination of vascular patterning and organ polarity
CC (PubMed:10559440, PubMed:11169198, PubMed:11402186, PubMed:15111711,
CC PubMed:15598805, PubMed:7555701). Directly regulates the expression of
CC AGO10, ZPR1, ZPR2, ZPR3 and ZPR4 (PubMed:22781836). Required to
CC regulate adaxial-abaxial polarity and leaf axial patterning
CC (PubMed:20807212). {ECO:0000269|PubMed:10559440,
CC ECO:0000269|PubMed:11169198, ECO:0000269|PubMed:11402186,
CC ECO:0000269|PubMed:15111711, ECO:0000269|PubMed:15598805,
CC ECO:0000269|PubMed:20807212, ECO:0000269|PubMed:22781836,
CC ECO:0000269|PubMed:7555701}.
CC -!- SUBUNIT: Homodimer (Probable). Heterodimer with ZPR1, ZPR2, ZPR3 or
CC ZPR4 (PubMed:18055602). Interacts with ESR1 and ESR2 (PubMed:17376809).
CC Interacts with ZPR1, ZPR2, ZPR3 and ZPR4 (PubMed:18055602,
CC PubMed:18408069). Heterodimerization with ZPR3 prevents DNA binding by
CC REV (PubMed:18055602). {ECO:0000269|PubMed:17376809,
CC ECO:0000269|PubMed:18055602, ECO:0000269|PubMed:18408069,
CC ECO:0000305|PubMed:18055602}.
CC -!- INTERACTION:
CC Q9SE43; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-3134035, EBI-4426557;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:10559440}.
CC -!- TISSUE SPECIFICITY: Expressed in the interfascicular regions of stem
CC and vascular bundles of young roots and leaves.
CC {ECO:0000269|PubMed:10559440}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the earliest stages and throughout
CC LSM initiation and development. In embryo development, expressed at the
CC heart stage until the 'walking stick' stage in the adaxial portion of
CC the cotyledon primordia, the shoot apical meristem (SAM) and the
CC vascular precursor cells of the hypocotyl and root. In developing
CC flowers, expressed first at stage 1 in the center of L3 layer and then
CC expands to the center of L2 and L1 layers. Expressed in the center of
CC flower meristem through stages 4 and 5. At stage 6, expressed in the
CC adaxial side of the carpel primordia and then on the adaxial carpel
CC face. {ECO:0000269|PubMed:11169198}.
CC -!- INDUCTION: By auxin. Repressed by ZPR and miR165. Induced by DOF5.1
CC (PubMed:20807212). {ECO:0000269|PubMed:15111711,
CC ECO:0000269|PubMed:17237362, ECO:0000269|PubMed:20807212}.
CC -!- DISRUPTION PHENOTYPE: Plants display strongly reduced auxin polar
CC transport in inflorescence stems and hypocotyls. This phenotype is
CC probably due to altered cell differentiation and morphology
CC (PubMed:11402186). Repression by miR165 suppresses DOF5.1 over-
CC expression mediated upward-curling phenotype (PubMed:20807212).
CC {ECO:0000269|PubMed:11402186, ECO:0000269|PubMed:20807212}.
CC -!- SIMILARITY: Belongs to the HD-ZIP homeobox family. Class III subfamily.
CC {ECO:0000305}.
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DR EMBL; AF188994; AAF15262.2; -; mRNA.
DR EMBL; AY170127; AAO11835.1; -; Genomic_DNA.
DR EMBL; AF233592; AAF42938.1; -; mRNA.
DR EMBL; AB005246; BAB09842.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97367.1; -; Genomic_DNA.
DR EMBL; AK229564; BAF01416.1; -; mRNA.
DR EMBL; EF598684; ABR09120.1; -; Genomic_DNA.
DR EMBL; EF598685; ABR09121.1; -; Genomic_DNA.
DR EMBL; EF598686; ABR09122.1; -; Genomic_DNA.
DR EMBL; EF598687; ABR09123.1; -; Genomic_DNA.
DR EMBL; EF598688; ABR09124.1; -; Genomic_DNA.
DR EMBL; EF598689; ABR09125.1; -; Genomic_DNA.
DR EMBL; EF598690; ABR09126.1; -; Genomic_DNA.
DR EMBL; EF598691; ABR09127.1; -; Genomic_DNA.
DR EMBL; EF598692; ABR09128.1; -; Genomic_DNA.
DR EMBL; EF598693; ABR09129.1; -; Genomic_DNA.
DR EMBL; EF598694; ABR09130.1; -; Genomic_DNA.
DR EMBL; EF598695; ABR09131.1; -; Genomic_DNA.
DR EMBL; EF598696; ABR09132.1; -; Genomic_DNA.
DR EMBL; EF598697; ABR09133.1; -; Genomic_DNA.
DR EMBL; EF598698; ABR09134.1; -; Genomic_DNA.
DR EMBL; EF598699; ABR09135.1; -; Genomic_DNA.
DR EMBL; EF598700; ABR09136.1; -; Genomic_DNA.
DR EMBL; EF598701; ABR09137.1; -; Genomic_DNA.
DR EMBL; EF598702; ABR09138.1; -; Genomic_DNA.
DR EMBL; EF598703; ABR09139.1; -; Genomic_DNA.
DR EMBL; EF598704; ABR09140.1; -; Genomic_DNA.
DR EMBL; EF598705; ABR09141.1; -; Genomic_DNA.
DR EMBL; EF598706; ABR09142.1; -; Genomic_DNA.
DR RefSeq; NP_200877.1; NM_125462.4.
DR AlphaFoldDB; Q9SE43; -.
DR SMR; Q9SE43; -.
DR BioGRID; 21434; 5.
DR IntAct; Q9SE43; 1.
DR STRING; 3702.AT5G60690.1; -.
DR PaxDb; Q9SE43; -.
DR PRIDE; Q9SE43; -.
DR ProteomicsDB; 236853; -.
DR EnsemblPlants; AT5G60690.1; AT5G60690.1; AT5G60690.
DR GeneID; 836190; -.
DR Gramene; AT5G60690.1; AT5G60690.1; AT5G60690.
DR KEGG; ath:AT5G60690; -.
DR Araport; AT5G60690; -.
DR TAIR; locus:2175856; AT5G60690.
DR eggNOG; ENOG502QRJM; Eukaryota.
DR HOGENOM; CLU_012517_0_0_1; -.
DR OMA; IPLAHTV; -.
DR OrthoDB; 165228at2759; -.
DR PhylomeDB; Q9SE43; -.
DR PRO; PR:Q9SE43; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SE43; baseline and differential.
DR Genevisible; Q9SE43; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0008289; F:lipid binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IMP:TAIR.
DR GO; GO:0009855; P:determination of bilateral symmetry; IMP:TAIR.
DR GO; GO:0010014; P:meristem initiation; IMP:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IMP:UniProtKB.
DR GO; GO:0009956; P:radial pattern formation; IMP:TAIR.
DR GO; GO:0010051; P:xylem and phloem pattern formation; IMP:TAIR.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 3.30.530.20; -; 1.
DR InterPro; IPR044830; HD-Zip_III.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR013978; MEKHLA.
DR InterPro; IPR023393; START-like_dom_sf.
DR InterPro; IPR002913; START_lipid-bd_dom.
DR PANTHER; PTHR45950; PTHR45950; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF08670; MEKHLA; 1.
DR Pfam; PF01852; START; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00234; START; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50848; START; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Differentiation; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..842
FT /note="Homeobox-leucine zipper protein REVOLUTA"
FT /id="PRO_0000331736"
FT DOMAIN 151..379
FT /note="START"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00197"
FT DNA_BIND 22..85
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 90..121
FT /evidence="ECO:0000255"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 624..625
FT /note="SF -> TS (in strain: cv. Ag-0)"
FT VARIANT 625..627
FT /note="FSS -> SSI (in strain: cv. C24)"
FT VARIANT 625
FT /note="F -> S (in strain: cv. Br-0, cv. Ct-1, cv. Edi-0,
FT cv. Ga-0, cv. Landsberg erecta, cv. Ll-0, cv. Lz-0, cv. Ms-
FT 0, cv. Sorbo, cv. Tu-1, cv. Wa-1 and cv. Wassilewskija)"
FT /evidence="ECO:0000269|PubMed:17435248"
FT VARIANT 630
FT /note="S -> L (in strain: cv. Nd-1)"
FT /evidence="ECO:0000269|PubMed:17435248"
FT MUTAGEN 190
FT /note="P->L: In avb1; gain of function. Transformation of
FT the collateral vascular bundles into amphivasal bundles and
FT disruption of the ring-like arrangement of vascular bundles
FT in the stele."
FT /evidence="ECO:0000269|PubMed:15111711"
SQ SEQUENCE 842 AA; 92438 MW; 6B546D2A87046EAA CRC64;
MEMAVANHRE RSSDSMNRHL DSSGKYVRYT AEQVEALERV YAECPKPSSL RRQQLIRECS
ILANIEPKQI KVWFQNRRCR DKQRKEASRL QSVNRKLSAM NKLLMEENDR LQKQVSQLVC
ENGYMKQQLT TVVNDPSCES VVTTPQHSLR DANSPAGLLS IAEETLAEFL SKATGTAVDW
VQMPGMKPGP DSVGIFAISQ RCNGVAARAC GLVSLEPMKI AEILKDRPSW FRDCRSLEVF
TMFPAGNGGT IELVYMQTYA PTTLAPARDF WTLRYTTSLD NGSFVVCERS LSGSGAGPNA
ASASQFVRAE MLSSGYLIRP CDGGGSIIHI VDHLNLEAWS VPDVLRPLYE SSKVVAQKMT
ISALRYIRQL AQESNGEVVY GLGRQPAVLR TFSQRLSRGF NDAVNGFGDD GWSTMHCDGA
EDIIVAINST KHLNNISNSL SFLGGVLCAK ASMLLQNVPP AVLIRFLREH RSEWADFNVD
AYSAATLKAG SFAYPGMRPT RFTGSQIIMP LGHTIEHEEM LEVVRLEGHS LAQEDAFMSR
DVHLLQICTG IDENAVGACS ELIFAPINEM FPDDAPLVPS GFRVIPVDAK TGDVQDLLTA
NHRTLDLTSS LEVGPSPENA SGNSFSSSSS RCILTIAFQF PFENNLQENV AGMACQYVRS
VISSVQRVAM AISPSGISPS LGSKLSPGSP EAVTLAQWIS QSYSHHLGSE LLTIDSLGSD
DSVLKLLWDH QDAILCCSLK PQPVFMFANQ AGLDMLETTL VALQDITLEK IFDESGRKAI
CSDFAKLMQQ GFACLPSGIC VSTMGRHVSY EQAVAWKVFA ASEENNNNLH CLAFSFVNWS
FV
