REV_BIV29
ID REV_BIV29 Reviewed; 186 AA.
AC P24097; Q04143; Q65598;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 23-FEB-2022, entry version 58.
DE RecName: Full=Protein Rev;
GN Name=rev;
OS Bovine immunodeficiency virus (strain R29) (BIV) (Bovine
OS immunodeficiency-like virus).
OC Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX NCBI_TaxID=417296;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate R29-106, and Isolate R29-127;
RX PubMed=2183467; DOI=10.1016/0042-6822(90)90424-p;
RA Garvey K.J., Oberste M.S., Elser J.E., Braun M.J., Gonda M.A.;
RT "Nucleotide sequence and genome organization of biologically active
RT proviruses of the bovine immunodeficiency-like virus.";
RL Virology 175:391-409(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1645801; DOI=10.1128/jvi.65.7.3932-3937.1991;
RA Oberste M.S., Greenwood J.D., Gonda M.A.;
RT "Analysis of the transcription pattern and mapping of the putative rev and
RT env splice junctions of bovine immunodeficiency-like virus.";
RL J. Virol. 65:3932-3937(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Isolate R29-Nadin;
RA Nadin-Davis S.A., Chang S.C., Roth J.A., Carpenter S.;
RT "Isolation and characterization of cDNAs encoding rev and tat of bovine
RT immunodeficiency-like virus.";
RL Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION, AND SUBCELLULAR LOCATION.
RX PubMed=8411341; DOI=10.1128/jvi.67.11.6395-6405.1993;
RA Oberste M.S., Williamson J.C., Greenwood J.D., Nagashima K., Copeland T.D.,
RA Gonda M.A.;
RT "Characterization of bovine immunodeficiency virus rev cDNAs and
RT identification and subcellular localization of the Rev protein.";
RL J. Virol. 67:6395-6405(1993).
CC -!- FUNCTION: Escorts unspliced or incompletely spliced viral pre-mRNAs
CC (late transcripts) out of the nucleus of infected cells. These pre-
CC mRNAs carry a recognition sequence called Rev responsive element (RRE)
CC located in the env gene, that is not present in fully spliced viral
CC mRNAs (early transcripts). This function is essential since most viral
CC proteins are translated from unspliced or partially spliced pre-mRNAs
CC which cannot exit the nucleus by the pathway used by fully processed
CC cellular mRNAs (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimer; when bound to the RRE. Multimeric assembly is
CC essential for activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus {ECO:0000250}. Host
CC cytoplasm {ECO:0000250}. Note=The presence of both nuclear import and
CC nuclear export signals leads to continuous shuttling between the
CC nucleus and cytoplasm. {ECO:0000250}.
CC -!- DOMAIN: The RNA-binding motif binds to the RRE present in incompletely
CC spliced viral pre-mRNAs. This region also contains the NLS which
CC mediates nuclear localization. These overlapping functions prevent Rev
CC bound to RRE from undesirable return to the nucleus. When Rev binds the
CC RRE, the NLS becomes masked while the NES remains accessible (By
CC similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:8411341}.
CC -!- MISCELLANEOUS: The sequence shown is that of isolate R29-127.
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DR EMBL; M32690; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; M74712; AAA42772.1; -; mRNA.
DR EMBL; L06504; AAA42774.1; -; mRNA.
DR PIR; H34742; VKLJBT.
DR SMR; P24097; -.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR InterPro; IPR008411; BIV_Surface_Envelope.
DR Pfam; PF05858; BIV_Env; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Host cytoplasm; Host nucleus; mRNA transport; Phosphoprotein;
KW RNA-binding; Transport.
FT CHAIN 1..186
FT /note="Protein Rev"
FT /id="PRO_0000085473"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 44..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 121..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 85..120
FT /evidence="ECO:0000255"
FT MOTIF 73..97
FT /note="Nuclear localization signal and RNA-binding (RRE)"
FT /evidence="ECO:0000250"
FT MOTIF 102..115
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 123..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 14
FT /note="G -> E (in strain: Isolate R29-Nadin)"
FT VARIANT 24
FT /note="E -> K (in strain: Isolate R29-Nadin)"
FT VARIANT 57
FT /note="E -> K (in strain: Isolate R29-Nadin)"
FT VARIANT 63
FT /note="G -> D (in strain: Isolate R29-Nadin)"
FT VARIANT 113
FT /note="E -> K (in strain: Isolate R29-Nadin)"
SQ SEQUENCE 186 AA; 20844 MW; B3A0D4F25823EC26 CRC64;
MDQDLDRAER GERGGGSEEL LQEEINEGRL TAREALQTWI NNDSPRYVKK LRQGQPELPT
SPGGGGGRGH RARKLPGERR PGFWKSLREL VEQNRRKQER RLSGLDRRIQ QLEDLVRHMS
LGSPDPSTPS ASVLSVNPPA QTPLGHLPPR SYFKLKRVDC GAGWDLRTTA APGLPICELD
WIQGTK
