REV_CAEVC
ID REV_CAEVC Reviewed; 133 AA.
AC P33460;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Protein Rev;
DE AltName: Full=Rev-C;
GN Name=rev;
OS Caprine arthritis encephalitis virus (strain Cork) (CAEV-Co).
OC Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX NCBI_TaxID=11661;
OH NCBI_TaxID=9925; Capra hircus (Goat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2171210; DOI=10.1016/0042-6822(90)90303-9;
RA Saltarelli M., Querat G., Konings D.A.M., Vigne R., Clements J.E.;
RT "Nucleotide sequence and transcriptional analysis of molecular clones of
RT CAEV which generate infectious virus.";
RL Virology 179:347-364(1990).
RN [2]
RP RNA-BINDING, AND MUTAGENESIS OF LYS-13; GLU-14; ASN-15; VAL-19; THR-20;
RP MET-21; 22-ASP--GLU-24; LYS-25; GLU-26; ARG-27; LYS-28; ARG-29;
RP 31-GLY--THR-23 AND 34-ALA--GLN-37.
RX PubMed=12606073; DOI=10.1016/s0168-1702(02)00311-8;
RA Abelson M.L., Schoborg R.V.;
RT "Characterization of the caprine arthritis encephalitis virus (CAEV) rev N-
RT terminal elements required for efficient interaction with the RRE.";
RL Virus Res. 92:23-35(2003).
CC -!- FUNCTION: Escorts unspliced or incompletely spliced viral pre-mRNAs
CC (late transcripts) out of the nucleus of infected cells. These pre-
CC mRNAs carry a recognition sequence called Rev responsive element (RRE)
CC located in the env gene, that is not present in fully spliced viral
CC mRNAs (early transcripts). This function is essential since most viral
CC proteins are translated from unspliced or partially spliced pre-mRNAs
CC which cannot exit the nucleus by the pathway used by fully processed
CC cellular mRNAs (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimer; when bound to the RRE. Multimeric assembly is
CC essential for activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus {ECO:0000250}. Host
CC cytoplasm {ECO:0000250}. Note=The presence of both nuclear import and
CC nuclear export signals leads to continuous shuttling between the
CC nucleus and cytoplasm. {ECO:0000250}.
CC -!- DOMAIN: The RNA-binding motif binds to the RRE present in incompletely
CC spliced viral pre-mRNAs. This region also contains the NLS which
CC mediates nuclear localization. These overlapping functions prevent Rev
CC bound to RRE from undesirable return to the nucleus. When Rev binds the
CC RRE, the NLS becomes masked while the NES remains accessible (By
CC similarity). {ECO:0000250}.
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DR EMBL; M33677; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR PIR; F45345; F45345.
DR SMR; P33460; -.
DR Proteomes; UP000203242; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host nucleus; mRNA transport; Reference proteome;
KW RNA-binding; Transport.
FT CHAIN 1..133
FT /note="Protein Rev"
FT /id="PRO_0000085475"
FT REGION 13..27
FT /note="RNA-binding (RRE)"
FT REGION 22..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 59..82
FT /note="Nuclear localization signal and RNA-binding (RRE)"
FT /evidence="ECO:0000250"
FT MOTIF 89..102
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250"
FT MUTAGEN 13
FT /note="K->A: Almost complete loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 14
FT /note="E->A: Almost complete loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 15
FT /note="N->A: Almost complete loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 19
FT /note="V->A: Partial loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 20
FT /note="T->A: Almost complete loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 21
FT /note="M->A: Almost complete loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 22..24
FT /note="DGE->AAA: Slight loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 25
FT /note="K->A: Slight loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 26
FT /note="E->A: Slight loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 27
FT /note="R->A: Partial loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 28
FT /note="K->A: No loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 29
FT /note="R->A: No loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
FT MUTAGEN 31..33
FT /note="GFT->AAA: No loss of RRE-binding."
FT MUTAGEN 34..37
FT /note="AGQQ->GAAA: Slight loss of RRE-binding."
FT /evidence="ECO:0000269|PubMed:12606073"
SQ SEQUENCE 133 AA; 15283 MW; 127DFB5B0002D44E CRC64;
MDAGARYMRL TGKENWVEVT MDGEKERKRE GFTAGQQDIQ NSKYPDIPTG HSHHGNKSRR
RRRKSGFWRW LRGIRQQRNK RKSDSTESLE PCLGALAELT LEGAMEKGPA EAARPSADDG
NLDKWMAWRT PQK
