ID   REV_HV2D1               Reviewed;         103 AA.
AC   P17754;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   02-JUN-2021, entry version 79.
DE   RecName: Full=Protein Rev;
DE   AltName: Full=Regulator of expression of viral proteins;
GN   Name=rev;
OS   Human immunodeficiency virus type 2 subtype A (isolate D194) (HIV-2).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX   NCBI_TaxID=11713;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2467304; DOI=10.1073/pnas.86.7.2383;
RA   Kuehnel H., von Briesen H., Dietrich U., Adamski M., Mix D., Biesert L.,
RA   Kreutz R., Immelmann A., Henco K., Meichsner C., Andreesen R.,
RA   Gelderblom H., Ruebsamen-Waigmann H.;
RT   "Molecular cloning of two west African human immunodeficiency virus type 2
RT   isolates that replicate well in macrophages: a Gambian isolate, from a
RT   patient with neurologic acquired immunodeficiency syndrome, and a highly
RT   divergent Ghanian isolate.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:2383-2387(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2235509; DOI=10.1093/nar/18.20.6142;
RA   Kuehnel H., Kreutz R., Ruebsamen-Waigmann H.;
RT   "Nucleotide sequence of HIV-2D194, an isolate from a Gambian case of
RT   'neuro-AIDS', which showed excellent growth in macrophages.";
RL   Nucleic Acids Res. 18:6142-6142(1990).
CC   -!- FUNCTION: Escorts unspliced or incompletely spliced viral pre-mRNAs
CC       (late transcripts) out of the nucleus of infected cells. These pre-
CC       mRNAs carry a recognition sequence called Rev responsive element (RRE)
CC       located in the env gene, that is not present in fully spliced viral
CC       mRNAs (early transcripts). This function is essential since most viral
CC       proteins are translated from unspliced or partially spliced pre-mRNAs
CC       which cannot exit the nucleus by the pathway used by fully processed
CC       cellular mRNAs (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homomultimer; when bound to the RRE. Multimeric assembly is
CC       essential for activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus, host nucleolus. Host cytoplasm.
CC       Note=The presence of both nuclear import and nuclear export signals
CC       leads to continuous shuttling between the nucleus and cytoplasm.
CC       {ECO:0000250}.
CC   -!- DOMAIN: The RNA-binding motif binds to the RRE, a stem-and-loop
CC       structure present in incompletely spliced viral pre-mRNAs. This region
CC       also contains the NLS which mediates nuclear localization. These
CC       overlapping functions prevent Rev bound to RRE from undesirable return
CC       to the nucleus. When Rev binds the RRE, the NLS becomes masked while
CC       the NES remains accessible (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: This isolate is from a Gambian case of 'neuro-AIDS'.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; J04542; AAA76846.1; -; Genomic_DNA.
DR   EMBL; X52223; CAA36470.1; -; Genomic_DNA.
DR   PIR; S12158; S12158.
DR   SMR; P17754; -.
DR   Proteomes; UP000007422; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000625; REV_protein.
DR   Pfam; PF00424; REV; 1.
PE   3: Inferred from homology;
KW   AIDS; Host cytoplasm; Host nucleus; mRNA transport; RNA-binding; Transport.
FT   CHAIN           1..103
FT                   /note="Protein Rev"
FT                   /id="PRO_0000085281"
FT   REGION          16..24
FT                   /note="Homomultimerization"
FT                   /evidence="ECO:0000250"
FT   REGION          80..103
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           35..49
FT                   /note="Nuclear localization signal and RNA-binding (RRE)"
FT                   /evidence="ECO:0000250"
FT   MOTIF           71..82
FT                   /note="Nuclear export signal and binding to XPO1"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        89..103
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   103 AA;  11963 MW;  607BEF9A5B36E7A0 CRC64;
     MRDRADEEGL QEKLRLIRLL HQINPYPHGQ GTASQRRNRR RRRRRQWFRL VALATKLHTI
     PDPPTDSPLD RAIQQLQGLT IQELPDPPTD LPESNSNQGL AET