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REXO1_YEAST
ID   REXO1_YEAST             Reviewed;         553 AA.
AC   P53331; D6VV53;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=RNA exonuclease 1;
DE            EC=3.1.-.-;
DE   AltName: Full=RNase H(70);
GN   Name=RNH70; Synonyms=REX1; OrderedLocusNames=YGR276C; ORFNames=G9381;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9133740;
RX   DOI=10.1002/(sici)1097-0061(19970330)13:4<365::aid-yea78>3.0.co;2-f;
RA   Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.;
RT   "The sequence of a 8 kb segment on the right arm of yeast chromosome VII
RT   identifies four new open reading frames and the genes for yTAFII145.";
RL   Yeast 13:365-368(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   PARTIAL PROTEIN SEQUENCE, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=10359084; DOI=10.1016/s0014-5793(99)00512-8;
RA   Frank P., Braunshofer-Reiter C., Karwan A., Grimm R., Wintersberger U.;
RT   "Purification of Saccharomyces cerevisiae RNase H(70) and identification of
RT   the corresponding gene.";
RL   FEBS Lett. 450:251-256(1999).
RN   [5]
RP   FUNCTION.
RX   PubMed=10716935; DOI=10.1093/emboj/19.6.1357;
RA   van Hoof A., Lennertz P., Parker R.;
RT   "Three conserved members of the RNase D family have unique and overlapping
RT   functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs
RT   in yeast.";
RL   EMBO J. 19:1357-1365(2000).
RN   [6]
RP   FUNCTION, AND MUTAGENESIS OF LEU-305.
RX   PubMed=16100378; DOI=10.1093/nar/gki772;
RA   Nariai M., Tanaka T., Okada T., Shirai C., Horigome C., Mizuta K.;
RT   "Synergistic defect in 60S ribosomal subunit assembly caused by a mutation
RT   of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S
RT   rRNA in Saccharomyces cerevisiae.";
RL   Nucleic Acids Res. 33:4553-4562(2005).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: 3' exoribonuclease required for 5S rRNA maturation and for
CC       the proper maturation of the 5' cistron of the tRNA-Arg3 dicistronic
CC       gene. Involved with REX2 in the maturation of the 5.8S rRNA, and with
CC       REX2 and REX3, in the 3' processing of the U5L snRNA.
CC       {ECO:0000269|PubMed:10359084, ECO:0000269|PubMed:10716935,
CC       ECO:0000269|PubMed:16100378}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10359084}.
CC   -!- SIMILARITY: Belongs to the REXO1/REXO3 family. {ECO:0000305}.
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DR   EMBL; X84098; CAA58898.1; -; Genomic_DNA.
DR   EMBL; Z73061; CAA97306.1; -; Genomic_DNA.
DR   EMBL; Z73062; CAA97308.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08364.1; -; Genomic_DNA.
DR   PIR; S64609; S64609.
DR   RefSeq; NP_011792.1; NM_001181405.1.
DR   AlphaFoldDB; P53331; -.
DR   BioGRID; 33526; 146.
DR   DIP; DIP-5564N; -.
DR   IntAct; P53331; 2.
DR   MINT; P53331; -.
DR   STRING; 4932.YGR276C; -.
DR   iPTMnet; P53331; -.
DR   MaxQB; P53331; -.
DR   PaxDb; P53331; -.
DR   PRIDE; P53331; -.
DR   EnsemblFungi; YGR276C_mRNA; YGR276C; YGR276C.
DR   GeneID; 853193; -.
DR   KEGG; sce:YGR276C; -.
DR   SGD; S000003508; RNH70.
DR   VEuPathDB; FungiDB:YGR276C; -.
DR   eggNOG; KOG2248; Eukaryota.
DR   HOGENOM; CLU_008679_2_1_1; -.
DR   InParanoid; P53331; -.
DR   OMA; HKAGPPF; -.
DR   BioCyc; YEAST:G3O-30941-MON; -.
DR   PRO; PR:P53331; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53331; protein.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; ISS:SGD.
DR   GO; GO:0000175; F:3'-5'-exoribonuclease activity; IMP:SGD.
DR   GO; GO:0004527; F:exonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IGI:SGD.
DR   GO; GO:0002107; P:generation of mature 3'-end of 5S rRNA generated by RNA polymerase III; IMP:SGD.
DR   GO; GO:0034415; P:tRNA 3'-trailer cleavage, exonucleolytic; IMP:SGD.
DR   GO; GO:0034476; P:U5 snRNA 3'-end processing; IGI:SGD.
DR   CDD; cd06145; REX1_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR034922; REX1-like_exo.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Exonuclease; Hydrolase; Nuclease;
KW   Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing; tRNA processing.
FT   CHAIN           1..553
FT                   /note="RNA exonuclease 1"
FT                   /id="PRO_0000202869"
FT   DOMAIN          225..373
FT                   /note="Exonuclease"
FT   COILED          167..194
FT                   /evidence="ECO:0000255"
FT   COILED          509..533
FT                   /evidence="ECO:0000255"
FT   MOD_RES         24
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MUTAGEN         305
FT                   /note="L->W: In REX1-1; impairs 5S rRNA maturation."
FT                   /evidence="ECO:0000269|PubMed:16100378"
SQ   SEQUENCE   553 AA;  62849 MW;  01AA7CD441F88914 CRC64;
     MQVEGPDTNF VSDLALGSKK RRLSKTSVQE DDHTNVVSEV NKNKKKKKAK PMTCTLLKSV
     VEKGIGIKDV RDMTQYLLQA ENNSPKWIDI CNRSSLQKMI VLFIPGLQPD DFENGKNTFN
     EISDDNFKYI PGEIASTFHT FPVMAPGSKM TLFSPYNSFI NVGLSKMEKI NKLKELQKKK
     KITINDLVLS EQQLVANDYP LDSGDTNFDT DWVQTVDFTH GGSHIFALDC EMCLSEQGLV
     LTRISLVNFD NEVIYEELVK PDVPIVDYLT RYSGITEEKL TVGAKKTLRE VQKDLLKIIS
     RSDILIGHSL QNDLKVMKLK HPLVVDTAII YHHKAGDPFK PSLKYLSETF LNKSIQNGEH
     DSVEDARACL ELTKLKILNG LAFGIGINTE NLFTKLHRFE VKTVLLNDMI IKNHTEDDSK
     GQLIRCVEDD ETWTHIHENL NKDVKLIVGR IKNLERSRNY NKKPRKETPS FDASMVLHDI
     GQHLTQLYEN ATPGTMILIM SGTGDTRPWN NLSTELEFIQ DKKERLDKRR EREPEIVEAI
     KLARGGVASF TVK
 
 
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