ID   REXO3_YEAST             Reviewed;         404 AA.
AC   Q12090; D6VYA7; Q7LI70;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 155.
DE   RecName: Full=RNA exonuclease 3;
DE            EC=3.1.-.-;
GN   Name=REX3; OrderedLocusNames=YLR107W; ORFNames=L2904;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 90840 / EAY235 / FY23;
RX   PubMed=9090053;
RX   DOI=10.1002/(sici)1097-0061(19970315)13:3<241::aid-yea61>3.0.co;2-#;
RA   Verhasselt P., Volckaert G.;
RT   "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of
RT   Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-
RT   Arg3 and 23 new open reading frames, among which several homologies to
RT   proteins involved in cell division control and to mammalian growth factors
RT   and other animal proteins are found.";
RL   Yeast 13:241-250(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=10716935; DOI=10.1093/emboj/19.6.1357;
RA   van Hoof A., Lennertz P., Parker R.;
RT   "Three conserved members of the RNase D family have unique and overlapping
RT   functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs
RT   in yeast.";
RL   EMBO J. 19:1357-1365(2000).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
CC   -!- FUNCTION: 3' to 5' exoribonuclease required for proper 3' end
CC       maturation of MRP RNA and of the U5L snRNA.
CC       {ECO:0000269|PubMed:10716935}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- SIMILARITY: Belongs to the REXO1/REXO3 family. {ECO:0000305}.
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DR   EMBL; X89514; CAA61685.1; -; Genomic_DNA.
DR   EMBL; U53876; AAB67549.1; -; Genomic_DNA.
DR   EMBL; U53878; AAB67561.1; -; Genomic_DNA.
DR   EMBL; Z73279; CAA97672.1; -; Genomic_DNA.
DR   EMBL; Z73280; CAA97675.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09423.1; -; Genomic_DNA.
DR   PIR; S64944; S64944.
DR   RefSeq; NP_013208.1; NM_001181994.1.
DR   AlphaFoldDB; Q12090; -.
DR   SMR; Q12090; -.
DR   BioGRID; 31380; 79.
DR   IntAct; Q12090; 1.
DR   STRING; 4932.YLR107W; -.
DR   MaxQB; Q12090; -.
DR   PaxDb; Q12090; -.
DR   PRIDE; Q12090; -.
DR   EnsemblFungi; YLR107W_mRNA; YLR107W; YLR107W.
DR   GeneID; 850797; -.
DR   KEGG; sce:YLR107W; -.
DR   SGD; S000004097; REX3.
DR   VEuPathDB; FungiDB:YLR107W; -.
DR   eggNOG; KOG2248; Eukaryota.
DR   GeneTree; ENSGT00940000169529; -.
DR   HOGENOM; CLU_022453_5_4_1; -.
DR   InParanoid; Q12090; -.
DR   OMA; IDCEMGF; -.
DR   BioCyc; YEAST:G3O-32255-MON; -.
DR   PRO; PR:Q12090; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; Q12090; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; ISS:SGD.
DR   GO; GO:0000175; F:3'-5'-exoribonuclease activity; IMP:SGD.
DR   GO; GO:0004527; F:exonuclease activity; IBA:GO_Central.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IGI:SGD.
DR   GO; GO:0043628; P:small regulatory ncRNA 3'-end processing; IMP:SGD.
DR   GO; GO:0034476; P:U5 snRNA 3'-end processing; IGI:SGD.
DR   CDD; cd06145; REX1_like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR034922; REX1-like_exo.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   SMART; SM00479; EXOIII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Exonuclease; Hydrolase; Nuclease; Nucleus; Reference proteome;
KW   rRNA processing.
FT   CHAIN           1..404
FT                   /note="RNA exonuclease 3"
FT                   /id="PRO_0000120937"
FT   DOMAIN          243..389
FT                   /note="Exonuclease"
SQ   SEQUENCE   404 AA;  45880 MW;  FF6DF994ECA69A5F CRC64;
     MGSLLRPVDL VNQPLGFQER YKILQKLFKQ LQKAYAHTKG TNIDLERLAT RLEVHVAKNS
     LSGQSYKFNM SILLRDVLKY KGDLSKIKIN GRPLKGAKPH LSSIGNANSI TTKSKAMEAL
     KALILDSKVL EKNGYIVKEM QNKTNDDNST QLYAPCLRCS SNFKKTDIME KTLCRYHPLK
     RIYNRDTKNH QYPCCGETTD SVSFLRLGCK TFFHHVFRGE SYDELCKISK FSSTDDIDGV
     ENVLSLDCEM AFTSLGYEMI RLTIVDFFTG KTLFDHVIQP IGDIVDLNSD FSGVHEIDRT
     NCPTYKEALD VFLSENLINK NSILIGHGLE NDLNVMRLFH NKVIDTAILY SRTKFKVSLK
     NLAFEVLSRK IQNGEHDSSQ DAIATMDVVK VKIGISPSQN KWEK