REXO4_YEAST
ID REXO4_YEAST Reviewed; 289 AA.
AC Q08237; D6W1Y8;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=RNA exonuclease 4;
DE EC=3.1.-.-;
GN Name=REX4; OrderedLocusNames=YOL080C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=12364601; DOI=10.1093/nar/gkf538;
RA Eppens N.A., Faber A.W., Rondaij M., Jahangir R.S., van Hemert S.,
RA Vos J.C., Venema J., Raue H.A.;
RT "Deletions in the S1 domain of Rrp5p cause processing at a novel site in
RT ITS1 of yeast pre-rRNA that depends on Rex4p.";
RL Nucleic Acids Res. 30:4222-4231(2002).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=15525710; DOI=10.1261/rna.7155904;
RA Faber A.W., Vos J.C., Vos H.R., Ghazal G., Elela S.A., Raue H.A.;
RT "The RNA catabolic enzymes Rex4p, Rnt1p, and Dbr1p show genetic interaction
RT with trans-acting factors involved in processing of ITS1 in Saccharomyces
RT cerevisiae pre-rRNA.";
RL RNA 10:1946-1956(2004).
CC -!- FUNCTION: Exoribonuclease involved in ribosome biosynthesis. Involved
CC in the processing of ITS1, the internal transcribed spacer localized
CC between the 18S and 5.8S rRNAs. {ECO:0000269|PubMed:12364601,
CC ECO:0000269|PubMed:15525710}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the REXO4 family. {ECO:0000305}.
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DR EMBL; Z74822; CAA99090.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10704.1; -; Genomic_DNA.
DR PIR; S66773; S66773.
DR RefSeq; NP_014561.1; NM_001183334.1.
DR AlphaFoldDB; Q08237; -.
DR SMR; Q08237; -.
DR BioGRID; 34322; 77.
DR DIP; DIP-5458N; -.
DR IntAct; Q08237; 3.
DR STRING; 4932.YOL080C; -.
DR MaxQB; Q08237; -.
DR PaxDb; Q08237; -.
DR PRIDE; Q08237; -.
DR EnsemblFungi; YOL080C_mRNA; YOL080C; YOL080C.
DR GeneID; 854075; -.
DR KEGG; sce:YOL080C; -.
DR SGD; S000005440; REX4.
DR VEuPathDB; FungiDB:YOL080C; -.
DR eggNOG; KOG2249; Eukaryota.
DR GeneTree; ENSGT00940000159607; -.
DR HOGENOM; CLU_022453_2_0_1; -.
DR InParanoid; Q08237; -.
DR OMA; TMLIYKS; -.
DR BioCyc; YEAST:G3O-33483-MON; -.
DR PRO; PR:Q08237; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08237; protein.
DR GO; GO:0005730; C:nucleolus; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0008408; F:3'-5' exonuclease activity; ISS:SGD.
DR GO; GO:0004527; F:exonuclease activity; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IGI:SGD.
DR GO; GO:0006364; P:rRNA processing; IGI:SGD.
DR CDD; cd06144; REX4_like; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR037431; REX4_DEDDh_dom.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW Exonuclease; Hydrolase; Nuclease; Nucleus; Reference proteome;
KW rRNA processing.
FT CHAIN 1..289
FT /note="RNA exonuclease 4"
FT /id="PRO_0000131702"
FT DOMAIN 121..273
FT /note="Exonuclease"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 289 AA; 32895 MW; B4DC2FA3BA1B7FCF CRC64;
MALSSNWQAL LASESNPTSN GKNKQSNRKI RNVKKVSKTV NVSSTTQYAP RKRKNGSKIM
DMVYNMNKEI SKHEKDKLEG KVFEFNPNKA NTSTTIKEPV KVGISEDTRI NSNKSKEIGK
YIAMDCEFVG VGPEGKESAL ARISIVNYFG HVVLDEFVKP REKVVEWRTW VSGIKPEHMK
NAITFKEAQK KTADILEGRI LVGHALKHDL EALMLSHPKS LLRDTSRHLP FRKLYAKGKT
PSLKKLTREV LKISIQEGEH SSVEDARATM LLYKKEKTEF EKIHRNTFN
