ID   REX_BACSU               Reviewed;         215 AA.
AC   O05521;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Redox-sensing transcriptional repressor Rex {ECO:0000255|HAMAP-Rule:MF_01131};
GN   Name=rex {ECO:0000255|HAMAP-Rule:MF_01131}; Synonyms=ydiH;
GN   OrderedLocusNames=BSU05970;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9202461; DOI=10.1099/00221287-143-6-1861;
RA   Sadaie Y., Yata K., Fujita M., Sagai H., Itaya M., Kasahara Y.,
RA   Ogasawara N.;
RT   "Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the
RT   Bacillus subtilis chromosome.";
RL   Microbiology 143:1861-1866(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Modulates transcription in response to changes in cellular
CC       NADH/NAD(+) redox state. {ECO:0000255|HAMAP-Rule:MF_01131}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01131}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory Rex family.
CC       {ECO:0000255|HAMAP-Rule:MF_01131}.
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DR   EMBL; D88802; BAA19721.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12416.1; -; Genomic_DNA.
DR   PIR; A69787; A69787.
DR   RefSeq; NP_388478.1; NC_000964.3.
DR   RefSeq; WP_003234073.1; NZ_JNCM01000032.1.
DR   PDB; 2VT2; X-ray; 2.30 A; A/B=1-215.
DR   PDB; 2VT3; X-ray; 2.00 A; A/B=1-215.
DR   PDBsum; 2VT2; -.
DR   PDBsum; 2VT3; -.
DR   AlphaFoldDB; O05521; -.
DR   SMR; O05521; -.
DR   STRING; 224308.BSU05970; -.
DR   PaxDb; O05521; -.
DR   PRIDE; O05521; -.
DR   EnsemblBacteria; CAB12416; CAB12416; BSU_05970.
DR   GeneID; 939871; -.
DR   KEGG; bsu:BSU05970; -.
DR   PATRIC; fig|224308.179.peg.642; -.
DR   eggNOG; COG2344; Bacteria.
DR   InParanoid; O05521; -.
DR   OMA; HEQRKAG; -.
DR   PhylomeDB; O05521; -.
DR   BioCyc; BSUB:BSU05970-MON; -.
DR   EvolutionaryTrace; O05521; -.
DR   PRO; PR:O05521; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0051775; P:response to redox state; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01131; Rex; 1.
DR   InterPro; IPR003781; CoA-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR009718; Rex_DNA-bd_C_dom.
DR   InterPro; IPR022876; Tscrpt_rep_Rex.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR35786; PTHR35786; 1.
DR   Pfam; PF02629; CoA_binding; 1.
DR   Pfam; PF06971; Put_DNA-bind_N; 1.
DR   SMART; SM00881; CoA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; NAD; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..215
FT                   /note="Redox-sensing transcriptional repressor Rex"
FT                   /id="PRO_0000097885"
FT   DNA_BIND        18..57
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT   BINDING         92..97
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT   HELIX           12..28
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           36..43
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           47..56
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   TURN            63..65
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           69..82
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          88..91
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           95..102
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          107..109
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          113..119
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   TURN            121..125
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          126..128
FT                   /evidence="ECO:0007829|PDB:2VT2"
FT   STRAND          131..135
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           136..138
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           139..142
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          148..151
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           155..167
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          172..175
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   STRAND          187..191
FT                   /evidence="ECO:0007829|PDB:2VT3"
FT   HELIX           194..208
FT                   /evidence="ECO:0007829|PDB:2VT3"
SQ   SEQUENCE   215 AA;  24092 MW;  29EA3C50FAD72B43 CRC64;
     MNKDQSKIPQ ATAKRLPLYY RFLKNLHASG KQRVSSAELS DAVKVDSATI RRDFSYFGAL
     GKKGYGYNVD YLLSFFRKTL DQDEMTDVIL IGVGNLGTAF LHYNFTKNNN TKISMAFDIN
     ESKIGTEVGG VPVYNLDDLE QHVKDESVAI LTVPAVAAQS ITDRLVALGI KGILNFTPAR
     LNVPEHIRIH HIDLAVELQS LVYFLKHYSV LEEIE