REX_THEAQ
ID REX_THEAQ Reviewed; 211 AA.
AC Q9X2V5;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Redox-sensing transcriptional repressor Rex {ECO:0000255|HAMAP-Rule:MF_01131};
GN Name=rex {ECO:0000255|HAMAP-Rule:MF_01131};
OS Thermus aquaticus.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=271;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-17, AND SUBUNIT.
RC STRAIN=ATCC 25104 / DSM 625 / JCM 10724 / NBRC 103206 / NCIMB 11243 / YT-1;
RX PubMed=10076001; DOI=10.1093/nar/27.7.1690;
RA Du X., Pene J.J.;
RT "Identification, cloning and expression of p25, an AT-rich DNA-binding
RT protein from the extreme thermophile, Thermus aquaticus YT-1.";
RL Nucleic Acids Res. 27:1690-1697(1999).
CC -!- FUNCTION: Modulates transcription in response to changes in cellular
CC NADH/NAD(+) redox state. {ECO:0000255|HAMAP-Rule:MF_01131}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01131,
CC ECO:0000269|PubMed:10076001}.
CC -!- INTERACTION:
CC Q9X2V5; Q9X2V5: rex; NbExp=3; IntAct=EBI-15532709, EBI-15532709;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01131}.
CC -!- SIMILARITY: Belongs to the transcriptional regulatory Rex family.
CC {ECO:0000255|HAMAP-Rule:MF_01131}.
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DR EMBL; AF061257; AAD22519.1; -; Genomic_DNA.
DR PDB; 1XCB; X-ray; 2.90 A; A/B/C/D/E/F/G=1-211.
DR PDBsum; 1XCB; -.
DR AlphaFoldDB; Q9X2V5; -.
DR SMR; Q9X2V5; -.
DR DIP; DIP-48428N; -.
DR EvolutionaryTrace; Q9X2V5; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0051775; P:response to redox state; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01131; Rex; 1.
DR InterPro; IPR003781; CoA-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR009718; Rex_DNA-bd_C_dom.
DR InterPro; IPR022876; Tscrpt_rep_Rex.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR35786; PTHR35786; 1.
DR Pfam; PF02629; CoA_binding; 1.
DR Pfam; PF06971; Put_DNA-bind_N; 1.
DR SMART; SM00881; CoA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; DNA-binding; NAD;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..211
FT /note="Redox-sensing transcriptional repressor Rex"
FT /id="PRO_0000097925"
FT DNA_BIND 13..52
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT BINDING 87..92
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT HELIX 5..21
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 22..25
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 31..38
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 42..50
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 64..74
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 75..78
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 81..87
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 90..95
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 103..111
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 115..119
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 138..140
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 150..162
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 166..170
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:1XCB"
FT STRAND 182..186
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 189..192
FT /evidence="ECO:0007829|PDB:1XCB"
FT HELIX 193..201
FT /evidence="ECO:0007829|PDB:1XCB"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:1XCB"
SQ SEQUENCE 211 AA; 23222 MW; A49C2755DDB1D7EC CRC64;
MKVPEAAISR LITYLRILEE LEAQGVHRTS SEQLGELAQV TAFQVRKDLS YFGSYGTRGV
GYTVPVLKRE LRHILGLNRK WGLCIVGMGR LGSALADYPG FGESFELRGF FDVDPEKVGR
PVRGGVIEHV DLLPQRVPGR IEIALLTVPR EAAQKAADLL VAAGIKGILN FAPVVLEVPK
EVAVENVDFL AGLTRLSFAI LNPKWREEMM G
