ID   RF12_METAC              Reviewed;         415 AA.
AC   Q8TS00;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Peptide chain release factor subunit 1-2 {ECO:0000255|HAMAP-Rule:MF_00424};
DE   AltName: Full=Translation termination factor aRF1 2 {ECO:0000255|HAMAP-Rule:MF_00424};
GN   Name=prf12 {ECO:0000255|HAMAP-Rule:MF_00424}; Synonyms=erf1;
GN   OrderedLocusNames=MA_1008;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Directs the termination of nascent peptide synthesis
CC       (translation) in response to the termination codons UAA, UAG and UGA.
CC       {ECO:0000255|HAMAP-Rule:MF_00424}.
CC   -!- SUBUNIT: Heterodimer of two subunits, one of which binds GTP.
CC       {ECO:0000255|HAMAP-Rule:MF_00424}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00424}.
CC   -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00424}.
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DR   EMBL; AE010299; AAM04439.1; -; Genomic_DNA.
DR   RefSeq; WP_011021044.1; NC_003552.1.
DR   AlphaFoldDB; Q8TS00; -.
DR   SMR; Q8TS00; -.
DR   STRING; 188937.MA_1008; -.
DR   EnsemblBacteria; AAM04439; AAM04439; MA_1008.
DR   GeneID; 1472898; -.
DR   KEGG; mac:MA_1008; -.
DR   HOGENOM; CLU_035759_3_0_2; -.
DR   InParanoid; Q8TS00; -.
DR   OMA; RIAIHDF; -.
DR   OrthoDB; 32191at2157; -.
DR   PhylomeDB; Q8TS00; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0018444; C:translation release factor complex; IBA:GO_Central.
DR   GO; GO:1990825; F:sequence-specific mRNA binding; IBA:GO_Central.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IBA:GO_Central.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.30.420.60; -; 1.
DR   Gene3D; 3.30.960.10; -; 1.
DR   HAMAP; MF_00424; Rel_fact_arch_1; 1.
DR   InterPro; IPR042226; eFR1_2_sf.
DR   InterPro; IPR005140; eRF1_1_Pelota.
DR   InterPro; IPR024049; eRF1_1_sf.
DR   InterPro; IPR005141; eRF1_2.
DR   InterPro; IPR005142; eRF1_3.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR020918; Peptide_chain-rel_aRF1.
DR   InterPro; IPR004403; Peptide_chain-rel_eRF1/aRF1.
DR   PANTHER; PTHR10113; PTHR10113; 1.
DR   Pfam; PF03463; eRF1_1; 1.
DR   Pfam; PF03464; eRF1_2; 1.
DR   Pfam; PF03465; eRF1_3; 1.
DR   SMART; SM01194; eRF1_1; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF55481; SSF55481; 1.
DR   TIGRFAMs; TIGR03676; aRF1/eRF1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..415
FT                   /note="Peptide chain release factor subunit 1-2"
FT                   /id="PRO_0000143173"
SQ   SEQUENCE   415 AA;  46721 MW;  C9396A9274DF420C CRC64;
     MAEYCTYEKY VFKKQLETLK SKNGRSTELI SLYIPPDKQI SDVTKHLREE HEQASNIISK
     LIRNNVQEAL HSLLAKLRSL HKIPENGIVY FTGTVDTGAN RTGMVNEVLF PPEPVADYIY
     RCDSVFYLEP LEEMLRECTT YGLLLLDLRE ATIGMLVGRQ TEVIKHLHST VPGKQRKGGQ
     SAHRFEQLRR IAIHDFYKRI GDATSEAFLE LDPAELKGIL IGGHSPTKEE FNEGGFLHYE
     LQKKVLGLFD TGYTDESGFS ELINAAEETL QSIDLLKQKK DMEIFFKEIA TESGKISYGE
     DNVRANLEIK AVDVLLLSEE LRAERVTLKC RVCGYENKRT RKWKTGEAVP AIGHCPECDS
     ELEVTDVIDT VGELSELADK GDARIAFIST DFDEGSQLMI AFGGIAAILR YNTGV