ID   RF1M_BOVIN              Reviewed;         447 AA.
AC   Q3MHI7;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Peptide chain release factor 1, mitochondrial;
DE            Short=MRF-1;
DE            Short=MtRF-1;
DE   Flags: Precursor;
GN   Name=MTRF1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial peptide chain release factor that directs the
CC       termination of translation in response to the peptide chain non-cognate
CC       termination stop codons AGG and AGA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- PTM: Methylation of glutamine in the GGQ triplet is conserved from
CC       bacteria to mammals. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; BC105224; AAI05225.1; -; mRNA.
DR   RefSeq; NP_001030185.1; NM_001035013.2.
DR   RefSeq; XP_005213621.1; XM_005213564.3.
DR   RefSeq; XP_005213623.1; XM_005213566.3.
DR   RefSeq; XP_010808973.1; XM_010810671.2.
DR   RefSeq; XP_015329240.1; XM_015473754.1.
DR   AlphaFoldDB; Q3MHI7; -.
DR   SMR; Q3MHI7; -.
DR   STRING; 9913.ENSBTAP00000016261; -.
DR   PaxDb; Q3MHI7; -.
DR   PRIDE; Q3MHI7; -.
DR   Ensembl; ENSBTAT00000075243; ENSBTAP00000072535; ENSBTAG00000012260.
DR   GeneID; 504754; -.
DR   KEGG; bta:504754; -.
DR   CTD; 9617; -.
DR   VEuPathDB; HostDB:ENSBTAG00000012260; -.
DR   VGNC; VGNC:31750; MTRF1.
DR   eggNOG; KOG2726; Eukaryota.
DR   GeneTree; ENSGT00940000156877; -.
DR   HOGENOM; CLU_036856_0_3_1; -.
DR   InParanoid; Q3MHI7; -.
DR   OMA; CHQDTRM; -.
DR   OrthoDB; 1313171at2759; -.
DR   TreeFam; TF313720; -.
DR   Proteomes; UP000009136; Chromosome 12.
DR   Bgee; ENSBTAG00000012260; Expressed in semen and 107 other tissues.
DR   ExpressionAtlas; Q3MHI7; baseline.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0003747; F:translation release factor activity; IEA:InterPro.
DR   GO; GO:0070126; P:mitochondrial translational termination; IBA:GO_Central.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   2: Evidence at transcript level;
KW   Methylation; Mitochondrion; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..447
FT                   /note="Peptide chain release factor 1, mitochondrial"
FT                   /id="PRO_0000261001"
FT   MOD_RES         315
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   447 AA;  52548 MW;  C5517158EF4103A8 CRC64;
     MNRRHLFAWL FRHLSLNGHL QCHVHRHSHQ LTQIPLDTRL WVFRRNRNHT VHRLLNKNCS
     RRYCHQDTSM LWKHKALQKY MEDLNKEYQT LDHCLHHISA SEGDRRSLTR RHAELAPLAV
     IYKEIQEAEQ AIEELESMCK SLNKQDEKQL QELALEERQT IAQKINMLYS ELFQSLLPKE
     KYDKNDVILE VTSGRTTGGD ICQQFTREIF DMYQNYSSYK HWRFELLNYT PADYGGLHHA
     AARISGDNVY KHLKYEGGIH RVQRIPEVGL SSRMQRIHTG TMSVIVLPHP DEVDVKVDPK
     DLRIDTFRAK GAGGQHVNTT DSAVRLVHIP TGLVVECQQE RSQIKNKEIA LRVLRARLYQ
     QIIEKDKCQQ RSARKLQVGT RAQSERIRTY NFTQDRVTDH RIAYEVRNIK EFLCGEKCLD
     QLIQRLLQSA DEEAITEFLD ENLKSVK