ID   RF1M_KLULA              Reviewed;         396 AA.
AC   P41767;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Peptide chain release factor 1, mitochondrial;
DE            Short=MRF-1;
DE            Short=MtRF-1;
DE   Flags: Precursor;
GN   Name=MRF1; OrderedLocusNames=KLLA0E14850g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=8662205; DOI=10.1007/s002940050095;
RA   Pel H.J., Rozenfeld S., Bolotin-Fukuhara M.;
RT   "The nuclear Kluyveromyces lactis MRF1 gene encodes a mitochondrial class I
RT   peptide chain release factor that is important for cell viability.";
RL   Curr. Genet. 30:19-28(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Mitochondrial peptide chain release factor that directs the
CC       termination of translation in response to the peptide chain termination
CC       codons UAA and UAG.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- PTM: Methylation of glutamine in the GGQ triplet is conserved from
CC       bacteria to mammals. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; U19586; AAB18362.1; -; Genomic_DNA.
DR   EMBL; CR382125; CAG99704.1; -; Genomic_DNA.
DR   PIR; S70354; S70354.
DR   RefSeq; XP_454617.1; XM_454617.1.
DR   AlphaFoldDB; P41767; -.
DR   SMR; P41767; -.
DR   STRING; 28985.XP_454617.1; -.
DR   PRIDE; P41767; -.
DR   EnsemblFungi; CAG99704; CAG99704; KLLA0_E14807g.
DR   GeneID; 2894283; -.
DR   KEGG; kla:KLLA0_E14807g; -.
DR   eggNOG; KOG2726; Eukaryota.
DR   HOGENOM; CLU_036856_0_4_1; -.
DR   InParanoid; P41767; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:EnsemblFungi.
DR   GO; GO:0003747; F:translation release factor activity; IEA:EnsemblFungi.
DR   GO; GO:0070126; P:mitochondrial translational termination; IEA:EnsemblFungi.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Methylation; Mitochondrion; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..396
FT                   /note="Peptide chain release factor 1, mitochondrial"
FT                   /id="PRO_0000030336"
FT   REGION          317..340
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        317..336
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         268
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   396 AA;  44995 MW;  EF29B564C969DCA3 CRC64;
     MISKLFGRGK FFRNVALQVR FNSSEAVEFR PLHSSLISRA ELYVNELREL EDLLSQGGSF
     DLEKQKNFAK LSTIVDSYSK YREEVNQYKE LQEILELDPS LREEAEADIA ALLPDLNKTG
     DSLLNKLLPP HPFADKPSIL ELRPGVGGSE AMIFTQDLLN MYINYANYHK WKWNLISKTE
     NASGSGVLEA ILNIDEPGSY DKLKFEAGVH RVQRVPATES KGRTHTSTAA VIVLPKMGEE
     SESDAYERTF KPDEIRIDVM RASGKGGQHV NTTDSAVRLT HYPSGIVISM QEERSQHRNK
     AKAFAILRAR LAEKERLEKE EKERNARKDQ VSTTDRSDKI RTYNYPQNRI TDHRCGFTLY
     DIEGVMKGER LDDVIDAMDA FSSEQKAKQL LQDMSA