RF1M_MOUSE
ID RF1M_MOUSE Reviewed; 446 AA.
AC Q8K126;
DT 13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Peptide chain release factor 1, mitochondrial;
DE Short=MRF-1;
DE Short=MtRF-1;
DE Flags: Precursor;
GN Name=Mtrf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mitochondrial peptide chain release factor that directs the
CC termination of translation in response to the peptide chain non-cognate
CC termination stop codons AGG and AGA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- PTM: Methylation of glutamine in the GGQ triplet is conserved from
CC bacteria to mammals. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
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DR EMBL; AK043981; BAC31724.1; -; mRNA.
DR EMBL; BC028898; AAH28898.1; -; mRNA.
DR CCDS; CCDS27297.1; -.
DR RefSeq; NP_666072.3; NM_145960.4.
DR AlphaFoldDB; Q8K126; -.
DR SMR; Q8K126; -.
DR IntAct; Q8K126; 1.
DR STRING; 10090.ENSMUSP00000022600; -.
DR iPTMnet; Q8K126; -.
DR PhosphoSitePlus; Q8K126; -.
DR MaxQB; Q8K126; -.
DR PaxDb; Q8K126; -.
DR PRIDE; Q8K126; -.
DR ProteomicsDB; 254918; -.
DR Antibodypedia; 23394; 159 antibodies from 23 providers.
DR Ensembl; ENSMUST00000022600; ENSMUSP00000022600; ENSMUSG00000022022.
DR GeneID; 211253; -.
DR KEGG; mmu:211253; -.
DR UCSC; uc007usw.1; mouse.
DR CTD; 9617; -.
DR MGI; MGI:2384815; Mtrf1.
DR VEuPathDB; HostDB:ENSMUSG00000022022; -.
DR eggNOG; KOG2726; Eukaryota.
DR GeneTree; ENSGT00940000156877; -.
DR HOGENOM; CLU_036856_0_3_1; -.
DR InParanoid; Q8K126; -.
DR OMA; CHQDTRM; -.
DR OrthoDB; 1313171at2759; -.
DR PhylomeDB; Q8K126; -.
DR TreeFam; TF313720; -.
DR BioGRID-ORCS; 211253; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q8K126; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8K126; protein.
DR Bgee; ENSMUSG00000022022; Expressed in heart left ventricle and 65 other tissues.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0003747; F:translation release factor activity; IEA:InterPro.
DR GO; GO:0070126; P:mitochondrial translational termination; IBA:GO_Central.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 2: Evidence at transcript level;
KW Methylation; Mitochondrion; Protein biosynthesis; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..446
FT /note="Peptide chain release factor 1, mitochondrial"
FT /id="PRO_0000030334"
FT MOD_RES 314
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 446 AA; 52458 MW; FC25AFF242025DE5 CRC64;
MSHHLCIWLF RNPFLRACPQ RHVFLSCQQF RQISLDTRPW NFRQKKTHVL YQLLNKSWSR
GCCHQGTRKL WKHKALQKYM EDLNKEYQTL DQCLQGISEN EGDRRALHRR HAQLAPLAAV
YQEIQEAEQA IEELESLCKS LNKQDEKQLQ ELVSEERQII DQKIHRLYSE LLERLVPKEK
YDWSDVILEV TSGRTTGGDI CQQFTREIFD MYQNYSYYKH WKFELLNYTP ADYGGLHHAA
ARISGDSVYK HLKYEGGIHR VQRIPEVGLS SRMQRIHTGT MSVIVLPQPD EVDVKVDPKD
LRVDTFRARG AGGQHVNTTD SAVRLVHIPT GLVVECQQER SQLKNKEIAL RVLRARLYQQ
IIEKDRCQQQ NARKLQVGTR AQSERIRTYN FTQDRVTDHR IAYEVRDIKE FLRGEKCLDQ
LIERLLQSAD EEAISEFLDE SLQSVK
