ID   AAE_RAUSE               Reviewed;         387 AA.
AC   Q3MKY2;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Acetylajmalan esterase {ECO:0000303|PubMed:16133216};
DE            EC=3.1.1.80;
DE   Flags: Precursor;
GN   Name=AAE {ECO:0000312|EMBL:AAW88320.1};
OS   Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Rauvolfiinae; Rauvolfia.
OX   NCBI_TaxID=4060;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAW88320.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=16133216; DOI=10.1007/s00425-005-0031-0;
RA   Ruppert M., Woll J., Giritch A., Genady E., Ma X., Stockigt J.;
RT   "Functional expression of an ajmaline pathway-specific esterase from
RT   Rauvolfia in a novel plant-virus expression system.";
RL   Planta 222:888-898(2005).
CC   -!- FUNCTION: Deacetylates 17-O-acetylajmaline and 17-O-acetylnorajmaline,
CC       but is inactive toward other acetylated alkaloids.
CC       {ECO:0000269|PubMed:16133216}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=17-O-acetylajmaline + H2O = acetate + ajmaline + H(+);
CC         Xref=Rhea:RHEA:22124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30089, ChEBI:CHEBI:58567, ChEBI:CHEBI:58679; EC=3.1.1.80;
CC         Evidence={ECO:0000269|PubMed:16133216};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=17-O-acetylnorajmaline + H2O = acetate + H(+) + norajmaline;
CC         Xref=Rhea:RHEA:23796, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30089, ChEBI:CHEBI:77618, ChEBI:CHEBI:77725; EC=3.1.1.80;
CC         Evidence={ECO:0000269|PubMed:16133216};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.7 mM for 17-O-acetylnorajmaline {ECO:0000269|PubMed:16133216};
CC         KM=0.12 mM for 17-O-acetylajmaline {ECO:0000269|PubMed:16133216};
CC       pH dependence:
CC         Optimum pH is 8.0. Half maximum activity is seen at pH 6.5 and 9.8.
CC         {ECO:0000269|PubMed:16133216};
CC       Temperature dependence:
CC         Optimum temperature is 53 degrees Celsius.
CC         {ECO:0000269|PubMed:16133216};
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000255}.
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DR   EMBL; AY762990; AAW88320.1; -; mRNA.
DR   AlphaFoldDB; Q3MKY2; -.
DR   SMR; Q3MKY2; -.
DR   KEGG; ag:AAW88320; -.
DR   BRENDA; 3.1.1.80; 5309.
DR   GO; GO:0033879; F:acetylajmaline esterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; Glycoprotein; Hydrolase; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..387
FT                   /note="Acetylajmalan esterase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000402567"
FT   ACT_SITE        36
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q00017"
FT   ACT_SITE        337
FT                   /evidence="ECO:0000250|UniProtKB:Q00017"
FT   ACT_SITE        340
FT                   /evidence="ECO:0000250|UniProtKB:Q00017"
FT   CARBOHYD        98
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        199
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        249
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        296
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   387 AA;  42729 MW;  0191272B6BE11EBE CRC64;
     MGFARLLHLV FSLLVFAGIT NGLICPFDSI YQLGDSFSDT GNLIRLPPDG PTFTAAHFPY
     GETFPGTPTG RCSDGRLIID FIATALNLPL LNPYLQQNVS FRHGVNFAVA GATALDRSFL
     AARGVQVSDI HSHLSAQLNW FRTYLGSICS TPKECSNKLK NALFILGNIG NNDVNYAFPN
     RTIEEIRAYV PFITEAVANA TREIIRLGGS RVIVPGIFPI GCVARNLNFL NFFPDGDKDD
     LGCLSSLNNL SIYFNSLFQR ALASLSIEFP QAVIIYADYY NAWRFLFRNG PALGSNSTSL
     LKCCCGIGGP YNYDPDRECG SRGVPVCPNP TQYIQWDGTH FTQAAYRRVA EYVIPGIIKA
     LKCSYSNIQP FLREGEGRQA LRLNERE