ID   RF1_BUCAI               Reviewed;         361 AA.
AC   P57268;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=BU171;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; BA000003; BAB12888.1; -; Genomic_DNA.
DR   RefSeq; NP_240002.1; NC_002528.1.
DR   RefSeq; WP_009874128.1; NC_002528.1.
DR   AlphaFoldDB; P57268; -.
DR   SMR; P57268; -.
DR   STRING; 107806.10038853; -.
DR   EnsemblBacteria; BAB12888; BAB12888; BAB12888.
DR   KEGG; buc:BU171; -.
DR   PATRIC; fig|107806.10.peg.182; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_6; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177646"
FT   MOD_RES         235
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   361 AA;  41262 MW;  B2F6B2603D15FB99 CRC64;
     MNNSILNKLK SLRNRYQEIE IMLTQKNVIS NRENLKTLSK EYLKLSEIIK YFIEWEKLEV
     DIENVNILLN DVEIQGMAEE ELYFFNKKKK ALEKKINQLL LPEDPNDKHS CFIEIRSATG
     GDESSIFAGE LFRMYLRYAE SYSWKVEIMN TSESEKGGFK EIIAKITGRG ACGRLKFESG
     GHRVQRVPET ESQGRIHTST CTVAVMPVTP KTEKEEINSS DLKIDTFRSS GAGGQHVNTT
     DSAIRITHIP TGNVVECQDE RSQHKNKAKA LSILSARVYA AKLEKDRQES SSMRKILLGT
     GERSDRNRTY NFPQNRITDH RINLSIYKLD EVLQGKLDLL IDPIIQEYQA DMLSSLSKSE
     S