ID   AAE_RAUVE               Reviewed;          46 AA.
AC   P86830;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Acetylajmalan esterase {ECO:0000250|UniProtKB:Q3MKY2};
DE            EC=3.1.1.80;
DE   Flags: Fragment;
GN   Name=AAE {ECO:0000250|UniProtKB:Q3MKY2};
OS   Rauvolfia verticillata (Common devil-pepper) (Dissolena verticillata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Rauvolfiinae; Rauvolfia.
OX   NCBI_TaxID=329875;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND CATALYTIC ACTIVITY.
RX   PubMed=16133216; DOI=10.1007/s00425-005-0031-0;
RA   Ruppert M., Woll J., Giritch A., Genady E., Ma X., Stockigt J.;
RT   "Functional expression of an ajmaline pathway-specific esterase from
RT   Rauvolfia in a novel plant-virus expression system.";
RL   Planta 222:888-898(2005).
CC   -!- FUNCTION: Deacetylates 17-O-acetylajmaline and 17-O-acetylnorajmaline,
CC       but is inactive toward other acetylated alkaloids.
CC       {ECO:0000250|UniProtKB:Q3MKY2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=17-O-acetylajmaline + H2O = acetate + ajmaline + H(+);
CC         Xref=Rhea:RHEA:22124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30089, ChEBI:CHEBI:58567, ChEBI:CHEBI:58679; EC=3.1.1.80;
CC         Evidence={ECO:0000250|UniProtKB:Q3MKY2, ECO:0000269|PubMed:16133216};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=17-O-acetylnorajmaline + H2O = acetate + H(+) + norajmaline;
CC         Xref=Rhea:RHEA:23796, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30089, ChEBI:CHEBI:77618, ChEBI:CHEBI:77725; EC=3.1.1.80;
CC         Evidence={ECO:0000250|UniProtKB:Q3MKY2, ECO:0000269|PubMed:16133216};
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P86830; -.
DR   GO; GO:0033879; F:acetylajmaline esterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; Direct protein sequencing; Glycoprotein; Hydrolase.
FT   CHAIN           <1..>46
FT                   /note="Acetylajmalan esterase"
FT                   /id="PRO_0000402568"
FT   CARBOHYD        39
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:16133216"
FT   NON_TER         46
FT                   /evidence="ECO:0000303|PubMed:16133216"
SQ   SEQUENCE   46 AA;  5242 MW;  7F9778EE939B4CBD CRC64;
     NALFILGNIG NNDVNYAFPD RAIEEIRFYV PFITEAVANA TREIIR