RF1_CLOBB
ID RF1_CLOBB Reviewed; 360 AA.
AC B2TJY5;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=CLL_A0483;
OS Clostridium botulinum (strain Eklund 17B / Type B).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=935198;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Eklund 17B / Type B;
RA Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA Smith T.J., Sutton G., Brettin T.S.;
RT "Complete sequence of Clostridium botulinum strain Eklund.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR EMBL; CP001056; ACD25129.1; -; Genomic_DNA.
DR RefSeq; WP_012425848.1; NC_018648.1.
DR AlphaFoldDB; B2TJY5; -.
DR SMR; B2TJY5; -.
DR EnsemblBacteria; ACD25129; ACD25129; CLL_A0483.
DR KEGG; cbk:CLL_A0483; -.
DR PATRIC; fig|935198.13.peg.438; -.
DR HOGENOM; CLU_036856_0_1_9; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 928964at2; -.
DR Proteomes; UP000001195; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..360
FT /note="Peptide chain release factor 1"
FT /id="PRO_1000093442"
FT MOD_RES 234
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ SEQUENCE 360 AA; 41032 MW; 96D8ADB3AA87ECA6 CRC64;
MLLDKLAFIE NKYDELSVKI SDPSIMQNQN EWRKLCKEQA DLEIIVNAYK EYKQVIEDLQ
VNKEMLSDES DREMKEMLNE EIASLSQREV ELEKEIQILL LPKDPNDDKN VFVEIRGGAG
GEEAALFAYN LFRMYTRYAE RQRWSTEIMS LNETDIGGFK EVVFMIKGNG AYSKLKYESG
VHRVQRVPDT ESSGRIHTST VTVAVLPEVD DVEIEIADKD VRIDVFRASG HGGQCVNTTD
SAVRITHLPS GLVVSCQDEK SQLKNKEKAM KVLRSRLFEK AEQERADGIA ADRKSQVGTG
DRSERIRTYN YPQGRITDHR IGLTLYKLDS FLDGDVQEMI NALITADQAE KMQKMGNSEM