ID   RF1_CLOD6               Reviewed;         354 AA.
AC   Q180Y2;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=CD630_34840;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; AM180355; CAJ70387.1; -; Genomic_DNA.
DR   RefSeq; WP_003421389.1; NZ_CP010905.2.
DR   RefSeq; YP_001090004.1; NC_009089.1.
DR   AlphaFoldDB; Q180Y2; -.
DR   SMR; Q180Y2; -.
DR   STRING; 272563.CD630_34840; -.
DR   PRIDE; Q180Y2; -.
DR   EnsemblBacteria; CAJ70387; CAJ70387; CD630_34840.
DR   GeneID; 66355945; -.
DR   KEGG; cdf:CD630_34840; -.
DR   KEGG; pdc:CDIF630_03795; -.
DR   PATRIC; fig|272563.120.peg.3682; -.
DR   eggNOG; COG0216; Bacteria.
DR   OMA; ISDHRVG; -.
DR   PhylomeDB; Q180Y2; -.
DR   BioCyc; PDIF272563:G12WB-3664-MON; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..354
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000263255"
FT   MOD_RES         233
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   354 AA;  40022 MW;  ACD26A5E8350164B CRC64;
     MLKKLEVLED TYKDLSEKIG DPDVINDQKV WQKYIKEHAD LEPIVMKYRE YKSVLDSIKE
     SKEILQEESD EELRELAKME LAEMEEKVAP LEEEIKILLL PKDPNDDKNV IVEIRGGAGG
     DEAALFAGDL FRMYSRYAER RRWKIELLSA SDTGVGGYKE VSFMIKGKGA YSRLKYESGV
     HRVQRIPSTE SGGRIHTSTS TVAVLPEVED VEVEINPNDL RIDVFRSSGN GGQSVNTTDS
     AVRVTHIPTG EVVSCQDGKS QLKNKEQALK ILKARLYDKA LAEQHKDIAA ERKSQVGTGD
     RSERIRTYNF PQGRISDHRI NLTLYKLDAF LDGDIDEMID ALITVDQTEK MTAI