RF1_CLOTE
ID RF1_CLOTE Reviewed; 355 AA.
AC Q898Y5;
DT 20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=CTC_00302;
OS Clostridium tetani (strain Massachusetts / E88).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=212717;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Massachusetts / E88;
RX PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA Gottschalk G.;
RT "The genome sequence of Clostridium tetani, the causative agent of tetanus
RT disease.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR EMBL; AE015927; AAO34944.1; -; Genomic_DNA.
DR RefSeq; WP_011098615.1; NC_004557.1.
DR AlphaFoldDB; Q898Y5; -.
DR SMR; Q898Y5; -.
DR STRING; 212717.CTC_00302; -.
DR PRIDE; Q898Y5; -.
DR EnsemblBacteria; AAO34944; AAO34944; CTC_00302.
DR GeneID; 64180417; -.
DR KEGG; ctc:CTC_00302; -.
DR HOGENOM; CLU_036856_0_1_9; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 928964at2; -.
DR Proteomes; UP000001412; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT CHAIN 1..355
FT /note="Peptide chain release factor 1"
FT /id="PRO_0000177662"
FT MOD_RES 233
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ SEQUENCE 355 AA; 40841 MW; 4BAD9361F9AABEFA CRC64;
MLDRLNFIEN KYEELSIKIS DPTVMQDQKE WQKLCKEHSD METIVTTYKE YKEVLQSIED
NKEMLKEDIE QELRDMVQED IKELEQRVQE LEQELKMLLV PKDPNDEKNV FIEIRAGAGG
DEAALFAANL FRMYTRYAER HNWKTEAVSV NETDIGGFKE IVFMVRGKGA YSRLKYESGV
HRVQRVPDTE SSGRIHTSTA TVAVLPEVED VDVEINQNDL RVDVYRASGH GGQCVNTTDS
AVRITHLPSG LVVTCQDEKS QLKNKEKAMK VLKSRLYDML ESERSASIAE DRKSQVGTGD
RSERIRTYNY PQGRVTDHRI GLTLYKLESF LDGDIEEMID GLITVEQSER MKDIS