ID   RF1_COXBU               Reviewed;         361 AA.
AC   P47849;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   09-MAY-2003, sequence version 2.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Peptide chain release factor 1;
DE            Short=RF-1;
GN   Name=prfA; OrderedLocusNames=CBU_1965;
OS   Coxiella burnetii (strain RSA 493 / Nine Mile phase I).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC   Coxiella.
OX   NCBI_TaxID=227377;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Nine Mile phase I;
RX   PubMed=8549730; DOI=10.1007/bf01719308;
RA   Willems H., Thiele D., Krauss H.;
RT   "Sequencing and linkage analysis of a Coxiella burnetii 2.1 kb NotI
RT   fragment.";
RL   Eur. J. Epidemiol. 11:559-561(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RSA 493 / Nine Mile phase I;
RX   PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA   Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C.,
RA   Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T.,
RA   Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M.,
RA   Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E.,
RA   Fraser C.M., Heidelberg J.F.;
RT   "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
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DR   EMBL; X78969; CAA55563.1; -; Genomic_DNA.
DR   EMBL; AE016828; AAO91454.1; -; Genomic_DNA.
DR   PIR; A61648; A61648.
DR   RefSeq; NP_820940.1; NC_002971.3.
DR   RefSeq; WP_005772919.1; NZ_CDBG01000001.1.
DR   AlphaFoldDB; P47849; -.
DR   SMR; P47849; -.
DR   STRING; 227377.CBU_1965; -.
DR   DNASU; 1209878; -.
DR   EnsemblBacteria; AAO91454; AAO91454; CBU_1965.
DR   GeneID; 1209878; -.
DR   KEGG; cbu:CBU_1965; -.
DR   PATRIC; fig|227377.7.peg.1953; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_6; -.
DR   OMA; ISDHRVG; -.
DR   Proteomes; UP000002671; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000177664"
FT   REGION          286..306
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        286..304
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         237
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        99
FT                   /note="K -> N (in Ref. 1; CAA55563)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   361 AA;  40763 MW;  EDB0A087C408F257 CRC64;
     MKPSLIEKLK TLTYRYSEIG GLLSDSTVIN DQDRYRELGK EYAQLEPIVK CFQQFQQNEK
     AIESAEEMQQ EKDPELRKLA EEELEQLTLK KEELEDQLKL LLVPKDPNDE RNVFLEIRAG
     TGGNEAAIFA GDLFRMYARY AETKGWRVNI VSAHEGEHGG FKEVIARVIG EGVYSQLKFE
     SGAHRVQRVP VTESQGRIHT SACTVAIMPE VDEIDQIKIN PAELRIDTFR ASGAGGQHVN
     RTDSAIRITH LPTGVVVECQ DERSQHKNKA RAMSLLQSKL LAAERAKQDQ EQAAKRKSLV
     GSGDRSERIR TYNFPQGRVT DHRINLTLYQ LDEVIEGDLD PVIGPLIREL QAEQLAELSG
     E