RF1_CYAP4
ID RF1_CYAP4 Reviewed; 364 AA.
AC B8HXV0;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN OrderedLocusNames=Cyan7425_1003;
OS Cyanothece sp. (strain PCC 7425 / ATCC 29141).
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales;
OC Cyanothecaceae; Cyanothece; unclassified Cyanothece.
OX NCBI_TaxID=395961;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7425 / ATCC 29141;
RX PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA Sherman L.A., Pakrasi H.B.;
RT "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT unicellular nitrogen-fixing Cyanobacteria.";
RL MBio 2:E214-E214(2011).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR EMBL; CP001344; ACL43389.1; -; Genomic_DNA.
DR RefSeq; WP_012626487.1; NC_011884.1.
DR AlphaFoldDB; B8HXV0; -.
DR SMR; B8HXV0; -.
DR STRING; 395961.Cyan7425_1003; -.
DR PRIDE; B8HXV0; -.
DR EnsemblBacteria; ACL43389; ACL43389; Cyan7425_1003.
DR KEGG; cyn:Cyan7425_1003; -.
DR eggNOG; COG0216; Bacteria.
DR HOGENOM; CLU_036856_0_1_3; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 928964at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..364
FT /note="Peptide chain release factor 1"
FT /id="PRO_1000193484"
FT REGION 291..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 239
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ SEQUENCE 364 AA; 41063 MW; C994D691A7BA1344 CRC64;
MAESYLLDKL KSVELTFHEL TRRLADPEVA TDPEEFQRVA KARASLEETV DTYDTWKTVQ
QHLAEARQIA REAASDPELQ EMAVQEVNEL TEKSAHLEQR LKILLLPRDP NDDKNIMLEI
RAGTGGDEAS IWAGDLVRLY SRYAESQRWR VKLVSESLGE MGGFKEAILE IQGEQVYSKL
KYEAGVHRVQ RVPATEASGR VHTSTATVAI MPEVDEVEVK IDPKDIELTT ARSGGAGGQN
VNKVETAVDL FHKPTGIRIF CTEERSQLQN KERAMQILRA KLYEMKLQEQ QSEITSRRRS
QVGTGSRSEK IRTYNYKDNR ATDHRLGQNF PLNQVLEGDI EGIIQACITQ DQQEQLAELA
AATA