RF1_DEIRA
ID RF1_DEIRA Reviewed; 375 AA.
AC P56905;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Peptide chain release factor 1;
DE Short=RF-1;
GN Name=prfA; OrderedLocusNames=DR_0976;
OS Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OC Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC Deinococcus.
OX NCBI_TaxID=243230;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC 9279 / R1 / VKM B-1422;
RX PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT R1.";
RL Science 286:1571-1577(1999).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
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DR EMBL; AE000513; AAF10553.1; -; Genomic_DNA.
DR PIR; C75452; C75452.
DR RefSeq; NP_294700.1; NC_001263.1.
DR RefSeq; WP_010887621.1; NZ_CP015081.1.
DR AlphaFoldDB; P56905; -.
DR SMR; P56905; -.
DR STRING; 243230.DR_0976; -.
DR EnsemblBacteria; AAF10553; AAF10553; DR_0976.
DR KEGG; dra:DR_0976; -.
DR PATRIC; fig|243230.17.peg.1163; -.
DR eggNOG; COG0216; Bacteria.
DR HOGENOM; CLU_036856_0_1_0; -.
DR InParanoid; P56905; -.
DR OMA; CHQDTRM; -.
DR OrthoDB; 928964at2; -.
DR Proteomes; UP000002524; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT CHAIN 1..375
FT /note="Peptide chain release factor 1"
FT /id="PRO_0000177665"
FT REGION 289..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..306
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 237
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 375 AA; 41126 MW; 61A5BB081A76831C CRC64;
MANMSRLNEL VSEFGLVERR LGDPQALADG REYARLTRRH RELLPLVTLV REREQAEADL
SGARELLQDP DMRDPDMKEL AQLEVGGLQA RLAEIEAELE VLLLPTDPDD GKDVILELRA
GAGGAEAGLF VMDLLRMYER YAAGLNLKLN VLDAAESDLG GASKVVAEVT GDFAFRALKW
ERGVHRVQRV PATESQGRIH TSTATVAVLP EAEPGEVNLD LSEVRIDVFR SQGAGGQGVN
TTDSAVRAVY RAGTPDEIMV ICQDGRSQIK NREKALQVLT ARLAERERAA REAQERQERA
SQVGSGDRSE KIRTYNYPQN RVTDHRLEGE DKNHPLDAVM AGGLAPVVSA LARAQREEQL
LAMSQEGAED QHGAA
