ID   RF1_DESHY               Reviewed;         355 AA.
AC   Q24ML9;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=DSY4934;
OS   Desulfitobacterium hafniense (strain Y51).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=138119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y51;
RX   PubMed=16513756; DOI=10.1128/jb.188.6.2262-2274.2006;
RA   Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA   Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT   "Complete genome sequence of the dehalorespiring bacterium
RT   Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT   ethenogenes 195.";
RL   J. Bacteriol. 188:2262-2274(2006).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE86723.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP008230; BAE86723.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_026199033.1; NC_007907.1.
DR   AlphaFoldDB; Q24ML9; -.
DR   SMR; Q24ML9; -.
DR   STRING; 138119.DSY4934; -.
DR   EnsemblBacteria; BAE86723; BAE86723; DSY4934.
DR   KEGG; dsy:DSY4934; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_9; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000001946; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..355
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000263267"
FT   MOD_RES         233
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   355 AA;  40083 MW;  2B0F62F25D38BC8F CRC64;
     MQRKLIEIER RYDELTQLLS DPEVIGNQSE WQKYAKAQAG MTDIVEAFRE YSEVVKHLEE
     TQGLLEEKLD PEFKEMAELE LEELTEKRDE LEEKMRILLL PKDPNDDKNV IMEIRAGAGG
     EEAALFAGDL YRMYTKYAEQ QGWRTELLSA SYTDIGGFKE IIFLIEGQGA YSKLKFESGV
     HRVQRVPTTE SGGRIHTSTT TVAVLPEAEE VDVAINPNDL RIDIFCSSGP GGQSVNTTQS
     AVRITHVPTG TVVSCQDEKS QLKNKEKALR VLRARLLERA QEEAMGELAS ERRSQVGTGD
     RSERIRTYNF PQGRVTDHRI GLTLHRLDSI LLGELDEVIN ALITTDQAER LKSEA