RF1_GEOSW
ID RF1_GEOSW Reviewed; 358 AA.
AC C5D9N4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=GWCH70_3322;
OS Geobacillus sp. (strain WCH70).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC unclassified Geobacillus.
OX NCBI_TaxID=471223;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WCH70;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA Brumm P., Mead D.A., Richardson P.;
RT "Complete sequence of chromosome of Geopacillus sp. WCH70.";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR EMBL; CP001638; ACS25962.1; -; Genomic_DNA.
DR RefSeq; WP_015865328.1; NC_012793.1.
DR AlphaFoldDB; C5D9N4; -.
DR SMR; C5D9N4; -.
DR STRING; 471223.GWCH70_3322; -.
DR EnsemblBacteria; ACS25962; ACS25962; GWCH70_3322.
DR KEGG; gwc:GWCH70_3322; -.
DR eggNOG; COG0216; Bacteria.
DR HOGENOM; CLU_036856_0_1_9; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 928964at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methylation; Protein biosynthesis.
FT CHAIN 1..358
FT /note="Peptide chain release factor 1"
FT /id="PRO_1000202696"
FT MOD_RES 233
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ SEQUENCE 358 AA; 40985 MW; 816E935CDC4DD850 CRC64;
MFERLEAVEE RYEKLNQLLM DPEVINDPKK LRDYSKEQSD LSETVQTYRE YKSVRKQLDE
AKAMLEEKLD PEMREMVKEE IDELEQKEEE LVNKLKILLL PKDPNDEKNV IMEIRAAAGG
EEAALFAGDL YRMYTRYAES QGWKTEVIEA SPTGLGGYKE IIFTIIGKGA YSKLKYENGA
HRVQRVPETE SGGRIHTSTA TVACLPEMEE IEVEINEKDI RVDTFASSGP GGQSVNTTMS
AVRLTHIPTG IVVTCQDEKS QIKNKEKAMK VLRARIYDKY QQEARAEYDQ TRKQAVGTGD
RSERIRTYNF PQNRVTDHRI GLTIQKLDQV LDGNLDEIIE ALILDDQSKK LEQANNEL