ID   RF1_GLOC7               Reviewed;         370 AA.
AC   B7KI17;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093};
GN   OrderedLocusNames=PCC7424_3733;
OS   Gloeothece citriformis (strain PCC 7424) (Cyanothece sp. (strain PCC
OS   7424)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Gloeothece; Gloeothece citriformis.
OX   NCBI_TaxID=65393;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7424;
RX   PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA   Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA   Sherman L.A., Pakrasi H.B.;
RT   "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT   unicellular nitrogen-fixing Cyanobacteria.";
RL   MBio 2:E214-E214(2011).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP001291; ACK72114.1; -; Genomic_DNA.
DR   RefSeq; WP_015955706.1; NC_011729.1.
DR   AlphaFoldDB; B7KI17; -.
DR   SMR; B7KI17; -.
DR   STRING; 65393.PCC7424_3733; -.
DR   EnsemblBacteria; ACK72114; ACK72114; PCC7424_3733.
DR   KEGG; cyc:PCC7424_3733; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_3; -.
DR   OMA; ISDHRVG; -.
DR   OrthoDB; 928964at2; -.
DR   Proteomes; UP000002384; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..370
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_1000117232"
FT   MOD_RES         239
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   370 AA;  41569 MW;  772F04E7388564E9 CRC64;
     MAEAYLLEKL KSVEQTYKEL TRRLADPDIA TNPDELQRVA KARSSLEETV NTYETWQKTK
     EEITGARQIL KEAGGDSEMR EMAALEVEEL EEQLEQLENK LKVLLLPRDP NDDKNIMLEI
     RAGTGGDEAS IWAGDLVRMY SRYAESQNWV VKLVSESLAD MGGFKEAILE IKGDQVYSKL
     KFEAGVHRVQ RVPVTEAGGR VHTSTATVAI MPEVDEVEVQ IDPKDIEVST ARSGGAGGQN
     VNKVETAVDL FHKPTGIRIF CTEERSQLQN RERAMQILRA KLYEMKLREQ QEAVSSMRRS
     QVGTGSRSEK IRTYNYKDNR VTDHRLGQNF SLVGALEGDI EVIIQSCIGK DQQERLAELA
     NSPETAPVLN