ID   RF1_LACAC               Reviewed;         361 AA.
AC   Q5FKZ0;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Peptide chain release factor 1 {ECO:0000255|HAMAP-Rule:MF_00093};
DE            Short=RF-1 {ECO:0000255|HAMAP-Rule:MF_00093};
GN   Name=prfA {ECO:0000255|HAMAP-Rule:MF_00093}; OrderedLocusNames=LBA0767;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC       translation in response to the peptide chain termination codons UAG and
CC       UAA. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF1. {ECO:0000255|HAMAP-Rule:MF_00093}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000255|HAMAP-Rule:MF_00093}.
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DR   EMBL; CP000033; AAV42634.1; -; Genomic_DNA.
DR   RefSeq; WP_003546735.1; NC_006814.3.
DR   RefSeq; YP_193665.1; NC_006814.3.
DR   AlphaFoldDB; Q5FKZ0; -.
DR   SMR; Q5FKZ0; -.
DR   STRING; 272621.LBA0767; -.
DR   PRIDE; Q5FKZ0; -.
DR   EnsemblBacteria; AAV42634; AAV42634; LBA0767.
DR   GeneID; 56942395; -.
DR   KEGG; lac:LBA0767; -.
DR   PATRIC; fig|272621.13.peg.730; -.
DR   eggNOG; COG0216; Bacteria.
DR   HOGENOM; CLU_036856_0_1_9; -.
DR   OMA; ISDHRVG; -.
DR   BioCyc; LACI272621:G1G49-784-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00093; Rel_fac_1; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004373; RF-1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
DR   TIGRFAMs; TIGR00019; prfA; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methylation; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..361
FT                   /note="Peptide chain release factor 1"
FT                   /id="PRO_0000263288"
FT   MOD_RES         236
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00093"
SQ   SEQUENCE   361 AA;  41523 MW;  2AF666D52159838F CRC64;
     MDKVMAQLES LVAHYEELQE MMADPEVIND TKRYMEISKE EADLREVVQK YKKYKENKKE
     IEDNKEIISN ETDSDLIEMA KEENAELEKE IPELEDQIKI LMLPKDPNDD KDIIMEIRGA
     AGGDEASLFA GDLLRMYEKY AERQNWNVSM IDSEPTEVGG YKRVAIMITG DKVYSKLKYE
     NGAHRVQRIP VTESQGRVHT STATVAVMPE YEQVDIDIDP KDIRVDVYRS SGAGGQHINK
     TSSAVRMTHL PTGIVVAMQD QRSQQQNREK AMQILKSRVY DYYESQNQAQ YDAKRKNAVG
     TGDRSERIRT YNYPQNRVTD HRIGLTLNKL DRVMNGELDE IIDALILYNQ TKQLEELADN
     A